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PCR-SSO typing for HLA-DRB alleles.

作者信息

Vaughan R W

机构信息

Molecular Immunogenetics, Guy's Hospital, London, U.K.

出版信息

Eur J Immunogenet. 1991 Feb-Apr;18(1-2):69-80. doi: 10.1111/j.1744-313x.1991.tb00007.x.

DOI:10.1111/j.1744-313x.1991.tb00007.x
PMID:1676912
Abstract

Polymerase chain reaction (PCR) amplification of the second exon of DRB genes from genomic DNA is described. Sequence-specific oligonucleotide (SSO) probes have been designed to allow for the typing of DRB alleles. The methods are described in detail and the results of a trial on both homozygous typing cells and restriction fragment length polymorphism (RFLP)-typed local caucasoid controls are given. The ease of use of this form of DRB typing is emphasized and potential complications are discussed.

摘要

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