PCR-SSO typing for HLA-DRB alleles.

Polymerase chain reaction (PCR) amplification of the second exon of DRB genes from genomic DNA is described. Sequence-specific oligonucleotide (SSO) probes have been designed to allow for the typing of DRB alleles. The methods are described in detail and the results of a trial on both homozygous typing cells and restriction fragment length polymorphism (RFLP)-typed local caucasoid controls are given. The ease of use of this form of DRB typing is emphasized and potential complications are discussed.