Komaki Hirokazu, Tanaka Takaaki, Chazono Masaaki, Kikuchi Takahiro
Department of Orthopaedic Surgery, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Tokyo, Japan.
Biomaterials. 2006 Oct;27(29):5118-26. doi: 10.1016/j.biomaterials.2006.05.031.
The objective of this study was to evaluate the effects of a complex of beta-tricalcium phosphate (beta-TCP) granules, collagen, and fibroblast growth factor-2 (FGF-2) on cortical bone repair in rabbits. Segmental bone defects of 5 mm in length were created in the middle of the tibial shaft. The defect was stabilized with a plate and screws, and was filled with 0.3 ml of a complex of beta-TCP granules and 5% collagen, with or without 200 microg of recombinant human fibroblast growth factor-2 (rhFGF-2). Bone regeneration and beta-TCP resorption were assessed by X-ray and micro-CT scanner. A three-point bending test was also performed. The results showed that the segmental bone defect was not only radiologically, but also mechanically healed with cortical bone 12 weeks after implantation of the complex with rhFGF-2. In contrast, after implantation of the complex without rhFGF-2, most of the defect was filled with beta-TCP and only a small amount of bone formation was found. These results suggest that resorption of beta-TCP is important for bone formation and may be promoted by FGF-2 in the beta-TCP implantation site. In addition, the complex of beta-TCP granules and collagen combined with rhFGF-2 provides a paste-like material that is easy to handle. This material may be of considerable use in the treatment of cortical bone defects.
本研究的目的是评估β-磷酸三钙（β-TCP）颗粒、胶原蛋白和成纤维细胞生长因子-2（FGF-2）复合物对兔皮质骨修复的影响。在胫骨干中部制造5毫米长的节段性骨缺损。用钢板和螺钉固定缺损，并用0.3毫升β-TCP颗粒与5%胶原蛋白的复合物填充，添加或不添加200微克重组人成纤维细胞生长因子-2（rhFGF-2）。通过X射线和微型CT扫描仪评估骨再生和β-TCP吸收情况。还进行了三点弯曲试验。结果显示，植入含rhFGF-2的复合物12周后，节段性骨缺损不仅在放射学上，而且在力学上均通过皮质骨愈合。相比之下，植入不含rhFGF-2的复合物后，大部分缺损被β-TCP填充，仅发现少量骨形成。这些结果表明，β-TCP的吸收对骨形成很重要，并且可能在β-TCP植入部位被FGF-2促进。此外，β-TCP颗粒与胶原蛋白的复合物与rhFGF-2结合提供了一种易于操作的糊状材料。这种材料在皮质骨缺损的治疗中可能有相当大的用途。
