Nohr D, Weihe E
Anatomical Institute, Johannes Gutenberg-University, Mainz, Federal Republic of Germany.
Brain Behav Immun. 1991 Mar;5(1):84-101. doi: 10.1016/0889-1591(91)90009-y.
This light microscopic immunohistochemical study investigates the distribution and target interrelations of nerve fibers in bronchus-associated lymphoid tissues (BALT) of rat and cat by using antisera against (1) the polyneuronal marker protein gene product 9.5 (PGP 9.5), (2) selected opioid and nonopioid peptides, and (3) the marker enzymes tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH). In both species, a similar distribution pattern of PGP, peptide, and catecholamine enzyme immunoreactive was observed. Anti-PGP 9.5 stained all nerve fibers (except some smaller, calcitonin gene-related peptide-immunoreactive (CGRP-ir) fibers presumably of the C-type) throughout the different compartments of BALT, e.g., under the epithelium, in the smooth muscle layer, along the vasculature, and between immune cells of BALT parenchyma. The distribution of fibers staining for peptides (substance P (SP), (CGRP), neuropeptide Y (NPY). Leu-enkephalin, Met-enkephalin-Arg-Gly-Leu) and/or the catecholamine enzymes was also not compartment-specific. However, the density of the different peptidergic fibers and those staining for the marker enzymes exhibited region- and target-specific variations, e.g., fibers, cocontaining substance P and CGRP were more ubiquitous in nonvascular regions than codistributed NPY-, TH-, and DBH-ir fibers, which clearly prevailed in perivascular plexus. Regularly, nerve fibers staining for any of the peptides and markers investigated formed close contacts with mast cells, cells of the macrophage/monocyte cell line (identified as ED1 + cells), and/or other lymphoid cells, although with different frequencies. We assume that the SP/CGRP innervation is mainly of primary sensory origin, while the NPY innervation is chiefly derived from postganglionic noradrenergic sympathetic neurons. The VIP/PHI component is most likely postganglionic cholinergic while the opioid component, apparently derived from the Proenkephalin precursor, could be of differential origin. We propose that the neuroimmune connections in BALT play a significant role in the regulation and/or modulation of physiological/pathophysiological mechanisms of the lung. BALT may also be an integral part of the psycho-neuro-immune axis.
本光镜免疫组织化学研究通过使用抗血清针对(1)多神经元标记蛋白基因产物9.5(PGP 9.5)、(2)选定的阿片样物质和非阿片样物质肽以及(3)标记酶酪氨酸羟化酶(TH)和多巴胺β-羟化酶(DBH)，来研究大鼠和猫支气管相关淋巴组织(BALT)中神经纤维的分布及其相互关系。在这两个物种中，观察到PGP、肽和儿茶酚胺酶免疫反应性的分布模式相似。抗PGP 9.5染色显示，在BALT的不同区域，如上皮下、平滑肌层、血管周围以及BALT实质的免疫细胞之间，所有神经纤维(除了一些较小的、可能属于C型的降钙素基因相关肽免疫反应性(CGRP-ir)纤维)均被染色。肽(物质P(SP)、CGRP、神经肽Y(NPY)、亮脑啡肽、甲硫脑啡肽-精氨酸-甘氨酸-亮氨酸)和/或儿茶酚胺酶染色的纤维分布也没有区域特异性。然而，不同肽能纤维和标记酶染色纤维的密度呈现出区域和靶点特异性变化，例如，同时含有物质P和CGRP的纤维在非血管区域比共分布的NPY-、TH-和DBH-ir纤维更普遍，而后者在血管周围丛中明显占优势。通常，针对所研究的任何肽和标记物染色的神经纤维与肥大细胞、巨噬细胞/单核细胞系细胞(鉴定为ED1+细胞)和/或其他淋巴细胞形成紧密接触，尽管频率不同。我们假设SP/CGRP神经支配主要来源于初级感觉神经，而NPY神经支配主要来自节后去甲肾上腺素能交感神经元。VIP/PHI成分很可能是节后胆碱能的，而阿片样物质成分显然来源于脑啡肽原前体，可能有不同的起源。我们提出，BALT中的神经免疫连接在肺的生理/病理生理机制的调节和/或调制中起重要作用。BALT也可能是心理-神经-免疫轴的一个组成部分。
