Inoue N, Magari S, Sakanaka M
Department of Anatomy, Osaka Medical College, Japan.
Lymphology. 1990 Sep;23(3):155-60.
The localization of neuropeptide Y (NPY), substance P (SP), calcitonin gene-related peptide (CGRP) and vasoactive intestinal polypeptide (VIP) in the nerve fibers of rat bronchus-associated lymphoid tissue (BALT) was investigated by light microscopic immunohistochemistry. Nerve fiber bundles revealing NPY-like immunoreactivity were shown to enter the BALT together with pulmonary artery branches. They frequently reached the central zone of the BALT to give rise to fine, tortuous fibers. On the other hand, nerve fibers immunoreactive for SP and CGRP seemed to distribute in the subepithelial zone of the BALT after dissociating from fiber networks in the walls of bronchi, although small numbers of SP and CGRP fibers were also seen in the BALT central zone. CGRP fibers formed a more intense network than SP fibers in the BALT. Scattered VIP fibers were found only in the subepithelial zone of the BALT. These findings not only suggest that the four kinds of peptidergic fibers act on BALT in multiple ways, but also that these neuropeptides may be involved in the control of mucosal immunity, lymphocyte migration and proliferation within the BALT.
应用光镜免疫组织化学方法，研究了神经肽Y（NPY）、P物质（SP）、降钙素基因相关肽（CGRP）及血管活性肠肽（VIP）在大鼠支气管相关淋巴组织（BALT）神经纤维中的定位。显示有NPY样免疫反应性的神经纤维束与肺动脉分支一起进入BALT。它们常到达BALT的中央区，发出纤细、曲折的纤维。另一方面，对SP和CGRP呈免疫反应的神经纤维在从支气管壁的纤维网络解离后，似乎分布在BALT的上皮下区，尽管在BALT中央区也可见少量SP和CGRP纤维。在BALT中，CGRP纤维形成的网络比SP纤维更密集。仅在BALT的上皮下区发现散在的VIP纤维。这些发现不仅提示这四种肽能纤维以多种方式作用于BALT，而且提示这些神经肽可能参与BALT内黏膜免疫、淋巴细胞迁移和增殖的调控。
