响尾蛇（响尾蛇属和小盾响尾蛇属）毒液生物活性的比较研究。

A comparative study of the biological activities of rattlesnake (genera Crotalus and Sistrurus) venoms.

Tan N H, Ponnudurai G

Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;98(2-3):455-61. doi: 10.1016/0742-8413(91)90233-j.

Abstract

The hemorrhagic, procoagulant, anticoagulant, protease, arginine ester hydrolase, phosphodiesterase, alkaline phosphomonoesterase, 5'-nucleotidase, hyaluronidase, phospholipase A and L-amino acid oxidase activities of 50 venom samples from 20 taxa of rattlesnake (genera Crotalus and Sistrurus) were examined. 2. The results show that notwithstanding individual variations in the biological activities of Crotalus venoms and the wide ranges of certain biological activities observed, there are some common characteristics at the genus and species levels. 3. The differences in biological activities of the venoms compared can be used for differentiation of the species. Particularly useful for this purpose are the thrombin-like enzyme, protease, arginine ester hydrolase, hemorrhagic and phospholipase A activities and kaolin-cephalin clotting time measurements.

摘要

对来自响尾蛇属（响尾蛇属和小响尾蛇属）20个分类单元的50份毒液样本的出血、促凝血、抗凝血、蛋白酶、精氨酸酯水解酶、磷酸二酯酶、碱性磷酸单酯酶、5'-核苷酸酶、透明质酸酶、磷脂酶A和L-氨基酸氧化酶活性进行了检测。2. 结果表明，尽管响尾蛇属毒液的生物活性存在个体差异，且观察到某些生物活性范围较广，但在属和种水平上仍有一些共同特征。3. 所比较毒液的生物活性差异可用于区分物种。为此特别有用的是类凝血酶、蛋白酶、精氨酸酯水解酶、出血和磷脂酶A活性以及高岭土-脑磷脂凝血时间测量。