Meng Zhaojing, Limbach Patrick A
Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA.
Brief Funct Genomic Proteomic. 2006 Mar;5(1):87-95. doi: 10.1093/bfgp/ell012. Epub 2006 Feb 27.
Ribonucleic acids (RNAs) are continuing to attract increased attention as they are found to play pivotal roles in biological systems. Just as genomics and proteomics have been enabled by the development of effective analytical techniques and instrumentation, the large-scale analysis of non-protein coding (nc)RNAs will benefit as new analytical methodologies, such as mass spectrometry (MS), are developed for their analysis. Mass spectrometry offers a number of advantages for RNA analysis arising from its ability to provide mass and sequence information starting with limited amounts of sample. This review will highlight recent developments in the field of MS that enable the characterization of RNA modification status, RNA tertiary structures, and ncRNA expression levels. These developments will also be placed in perspective of how MS of RNAs can help elucidate the link between the genome and proteome.
核糖核酸（RNAs）因其在生物系统中发挥关键作用而持续受到越来越多的关注。正如基因组学和蛋白质组学因有效分析技术和仪器的发展而得以实现一样，随着用于非蛋白质编码（nc）RNAs分析的新分析方法（如质谱（MS））的开发，对ncRNAs的大规模分析也将从中受益。质谱能够从有限量的样品开始提供质量和序列信息，这为RNA分析带来了诸多优势。本综述将重点介绍质谱领域的最新进展，这些进展能够实现对RNA修饰状态、RNA三级结构和ncRNA表达水平的表征。这些进展还将从RNA质谱如何有助于阐明基因组与蛋白质组之间的联系这一角度进行阐述。
