Cui Zhonghao, Chiu Norman H L, Wambua Dickson M
Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27402, USA.
Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27402, USA.
Noncoding RNA. 2017 Mar 16;3(1):13. doi: 10.3390/ncrna3010013.
microRNA (miRNA) are short endogenous non-coding RNA that play a crucial role in post-transcriptional gene regulation and have been implicated in the initiation and progression of 160+ human diseases. Excellent analytical methods have been developed for the measurement of miRNA by mass spectrometry. However, interpretation of mass spectrometric data has been an incapacitating bottleneck in miRNA identification. This study details the development of MicroRNA MultiTool, a software for the identification of miRNA from mass spectrometric data. The software includes capabilities such as miRNA search and mass calculator, modified miRNA mass calculator, and miRNA fragment search. MicroRNA MultiTool bridges the gap between experimental data and identification of miRNA by providing a rapid means of mass spectrometric data interpretation.
微小RNA（miRNA）是短链内源性非编码RNA，在转录后基因调控中起关键作用，并与160多种人类疾病的发生和发展有关。已经开发出了通过质谱法测量miRNA的优秀分析方法。然而，质谱数据的解读一直是miRNA鉴定中一个令人无能为力的瓶颈。本研究详细介绍了MicroRNA MultiTool的开发，这是一款用于从质谱数据中鉴定miRNA的软件。该软件具备诸如miRNA搜索和质量计算器、修饰的miRNA质量计算器以及miRNA片段搜索等功能。MicroRNA MultiTool通过提供一种快速解读质谱数据的方法，弥合了实验数据与miRNA鉴定之间的差距。
