Konstantinova S G, Russanova I E, Russanov E M
Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
Free Radic Res Commun. 1991;12-13 Pt 1:215-20. doi: 10.3109/10715769109145789.
The effects of cimetidine, ranitidine, histamine and histidine, as well as of their copper complexes, have been examined in an enzymic and chemical O2- generated systems. Copper complexes like CuZnSOD inhibited both the reduction of cytochrome c and NBT2+ in xanthine-xanthine oxidase systems, but their inhibitory action was due to a certain extent to the copper-induced inhibition of xanthine oxidase. EDTA abolished the inhibitory effect of all copper complexes studied. Luminol chemiluminescence in NADH2-PMS system was inhibited by CuZnSOD while it was enhanced by copper complexes. The copper-accelerating effect gradually increased up to about 1 microM Cu and decreased, reaching the control values up to 10 microM Cu. In the presence of low copper concentrations chemiluminescence was inhibited by CuZnSOD only, while in the presence of high copper concentrations it was inhibited by catalase and mannitol, but not by CuZnSOD. The ligands however, have been ineffective in the two O2- generated systems.
已在酶促和化学产生活性氧的系统中研究了西咪替丁、雷尼替丁、组胺和组氨酸及其铜配合物的作用。像铜锌超氧化物歧化酶（CuZnSOD）这样的铜配合物在黄嘌呤-黄嘌呤氧化酶系统中既抑制细胞色素c的还原，也抑制NBT2+的还原，但其抑制作用在一定程度上是由于铜对黄嘌呤氧化酶的诱导抑制。乙二胺四乙酸（EDTA）消除了所研究的所有铜配合物的抑制作用。在NADH2-PMS系统中，铜锌超氧化物歧化酶抑制鲁米诺化学发光，而铜配合物则增强化学发光。铜的促进作用在铜浓度约为1微摩尔时逐渐增强，在铜浓度达到10微摩尔时下降，直至达到对照值。在低铜浓度存在下，仅铜锌超氧化物歧化酶抑制化学发光，而在高铜浓度存在下，过氧化氢酶和甘露醇抑制化学发光，但铜锌超氧化物歧化酶不抑制。然而，这些配体在这两个产生活性氧的系统中均无作用。
