Bijloo G J, van der Goot H, Bast A, Timmerman H
Department of Pharmacochemistry, Vrije Universiteit, Amsterdam, The Netherlands.
J Inorg Biochem. 1990 Nov;40(3):237-44. doi: 10.1016/0162-0134(90)80057-5.
In a preliminary study we tested CuSO4.5H2O, (Cu(II]2[3,5-diisopropylsalicylate]4.2H2O and a number of copper complexes of substituted 1,10-phenanthrolines for superoxide anion dismutase activity. It appeared that this activity depends on the ligands involved and might be governed by the redox potential of the Cu(I) complex/Cu(II) complex couple. The strong superoxide anion dismutase activity of Cu(II)[DMP]2 complex can be expected considering its high redox potential. Rather surprisingly is the superoxide anion dismutase activity of the Cu(I)[DMP]2 complex since it involves oxidation to Cu(II)[DMP]2 complex. From regression analysis it was established that steric and field effects of the substituents of the investigated phenanthrolines play an important role in SOD activity and therefore it is concluded that complex formation is important for the superoxide dismutase-like activity.
在一项初步研究中，我们测试了五水硫酸铜（CuSO₄·5H₂O）、二水合双（3,5 - 二异丙基水杨酸根）铜（Ⅱ）（Cu(II)₂[3,5 - diisopropylsalicylate]₄·2H₂O）以及一些取代1,10 - 菲啰啉的铜配合物的超氧化物歧化酶活性。结果表明，这种活性取决于所涉及的配体，并且可能受Cu(I)配合物/Cu(II)配合物电对的氧化还原电位的控制。考虑到其较高的氧化还原电位，可以预期Cu(II)[DMP]₂配合物具有较强的超氧化物歧化酶活性。相当令人惊讶的是Cu(I)[DMP]₂配合物的超氧化物歧化酶活性，因为它涉及氧化为Cu(II)[DMP]₂配合物。通过回归分析确定，所研究菲啰啉取代基的空间和场效应在超氧化物歧化酶活性中起重要作用，因此得出结论，配合物的形成对于类超氧化物歧化酶活性很重要。
