亮抑蛋白酶肽，一种由侧孢芽孢杆菌BMI156 - 14F1产生的新型氨肽酶M抑制剂。I. 分类学、生产、分离、理化性质及生物活性。
Leuhistin, a new inhibitor of aminopeptidase M, produced by Bacillus laterosporus BMI156-14F1. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
作者信息
Aoyagi T, Yoshida S, Matsuda N, Ikeda T, Hamada M, Takeuchi T
机构信息
Institute of Microbial Chemistry, Tokyo, Japan.
出版信息
J Antibiot (Tokyo). 1991 Jun;44(6):573-8. doi: 10.7164/antibiotics.44.573.
Leuhistin has been isolated from the culture broth of Bacillus laterosporus BMI156-14F1 as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M (AP-M). It was purified by use of column chromatography on Sepabeads SP206, Amberlite IRC-50, MCI gel CHP-20P and Sephadex G-10 and then isolated as colorless needles. Leuhistin inhibits AP-M strongly and it also inhibits AP-A and AP-B weakly. It is competitive with the substrate, and the inhibition constant (Ki) was 2.3 x 10(-7) M.
亮抑酶肽是从侧孢芽孢杆菌BMI156 - 14F1的培养液中分离出来的，这是旨在寻找微生物产生的氨肽酶M（AP - M）抑制剂的项目的一部分。它通过在Sepabeads SP206、Amberlite IRC - 50、MCI凝胶CHP - 20P和Sephadex G - 10上进行柱色谱纯化，然后分离为无色针状晶体。亮抑酶肽强烈抑制AP - M，对AP - A和AP - B也有较弱的抑制作用。它与底物具有竞争性，抑制常数（Ki）为2.3×10⁻⁷ M。
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