Huang Cheng, Fan Jia, Zhou Jian, Liu Yin-kun, Cui Jie-feng
Institute of Liver Cancer, Department of Hepatic Surgery, Zhongshan Hospital of Fudan University, Shanghai 200032, China.
Zhonghua Wai Ke Za Zhi. 2006 Apr 1;44(7):445-9.
To investigate the effects of different clinicopathologic variables on serum protein fingerprint in hepatocellular carcinoma (HCC) patients.
Serum samples were collected from 112 HCC patients, Special serum protein or peptide spectra was determined by surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) measurement after treating the sample onto weak cation exchange (WCX2) protein chip for each case. The serum protein profiles were compared by BioMarker Wizard Software among the patients stratified according to gender, AFP, presence of portal vein tumor thrombus (PVTT), tumor size, tumor number, presence of cirrhosis, respectively.
According to serum protein fingerprints of 112 HCC patients, a total of 100 protein peaks were identified at the m/z value ranging from 1100 to 30,000. (1) Sixteen significant differential proteins were found between the groups of HCC with single tumor and those with multiple tumors (P < 0.01). (2) Only one significant differential protein was found between the groups of HCC with tumor size > 3 cm and those with tumor size <or= 3 cm, and 4 significant differential proteins between the groups of HCC with tumor size > 5 cm and those with tumor size <or= 5 cm, while 3 significant differential proteins between the groups of HCC with tumor size > 10 cm and those with tumor size <or= 10 cm (P < 0.01). (3) Sixteen significant differential proteins were found between the groups of macroscopic portal vein tumor thrombus (Ma-PVTT) and those without PVTT (N-PVTT); Only 2 significant differential protein were found between the groups of microscopic portal vein tumor thrombus (Mi-PVTT) and N-PVTT (P < 0.01); eight significant differential proteins were found between the groups of Ma-PVTT and Mi-PVTT (P < 0.01). (4) No significant differential protein was found when patients stratified according to gender, presence of cirrhosis and AFP.
PVTT, tumor number and tumor size had significant effects on serum protein fingerprint, while no significant effect on serum protein from gender, presence of cirrhosis and AFP. The most profound impact on the serum protein was attained when cutoff was chosen to be presence of Ma-PVTT compared to less effect from Mi-PVTT and 5cm for tumor size.
探讨不同临床病理变量对肝细胞癌（HCC）患者血清蛋白质指纹图谱的影响。
收集112例HCC患者的血清样本，将每份样本处理于弱阳离子交换（WCX2）蛋白质芯片上后，采用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）测定特殊血清蛋白质或肽谱。通过BioMarker Wizard软件对分别根据性别、甲胎蛋白（AFP）、门静脉癌栓（PVTT）、肿瘤大小、肿瘤数量、肝硬化情况分层的患者血清蛋白质谱进行比较。
根据112例HCC患者的血清蛋白质指纹图谱，在m/z值为1100至30000范围内共鉴定出100个蛋白质峰。（1）在单发肿瘤的HCC组和多发肿瘤的HCC组之间发现16种显著差异蛋白（P<0.01）。（2）肿瘤大小>3 cm的HCC组和肿瘤大小≤3 cm的HCC组之间仅发现1种显著差异蛋白，肿瘤大小>5 cm的HCC组和肿瘤大小≤5 cm的HCC组之间发现4种显著差异蛋白，而肿瘤大小>10 cm的HCC组和肿瘤大小≤10 cm的HCC组之间发现3种显著差异蛋白（P<0.01）。（3）在肉眼门静脉癌栓（Ma-PVTT）组和无PVTT（N-PVTT）组之间发现16种显著差异蛋白；在微小门静脉癌栓（Mi-PVTT）组和N-PVTT组之间仅发现2种显著差异蛋白（P<0.01）；在Ma-PVTT组和Mi-PVTT组之间发现8种显著差异蛋白（P<0.01）。（4）根据性别、肝硬化情况和AFP对患者进行分层时，未发现显著差异蛋白。
PVTT、肿瘤数量和肿瘤大小对血清蛋白质指纹图谱有显著影响，而性别、肝硬化情况和AFP对血清蛋白质无显著影响。与Mi-PVTT和肿瘤大小5 cm相比，选择Ma-PVTT的存在作为截断值时对血清蛋白质的影响最为显著。
