Qiu Ji-gang, Fan Jia, Liu Yin-kun, Zhou Jian, Qiu Shuang-jian, Dai Zhi, Kang Xiao-nan, Huang Cheng, Yang Peng-yuan, Tang Zhao-you
Liver Cancer Institute and Department of Liver Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Zhonghua Gan Zang Bing Za Zhi. 2007 Jul;15(7):498-502.
To screen low molecular weight protein biomarkers relevant to portal vein tumor thrombi (PVTT) in serum of hepatocellular carcinoma (HCC) patients.
Serum samples were obtained from 12 healthy volunteers, 12 HCC patients without PVTT and 12 HCC patients with PVTT. Using two-dimensional gel electrophoresis (2-DE) in which the second dimension was 16% SDS-PAGE, serum protein images of the 3 groups were analyzed by ImageMaster software. The differential protein spots were further identified by MALDI-TOF MS/MS.
Comparing the results using 12.5% SDS-PAGE gel, there were more protein bands (between 3 x 10(3) and 20 x 10(3)) and low molecular weight (MW) protein spots (less than 20 x 10(3)) were clearly shown in the 16% SDS-PAGE gel. Fifteen differential protein spots representing 5 proteins were found in the 3 groups by inter-class comparison and they were then identified. Compared with those in the healthy group, apolipoprotein A-I, lipoprotein CIII, transthyretin and DNA topoisomerase II were all down regulated in HCC groups and haptoglobin-2 was over expressed. All 5 proteins decreased more in the PVTT group than in the non-PVTT group.
The expression of low MW serum protein obviously changes in the beginning and in the progressive stage of HCC, and differentially expressed low MW proteins might be potential biomarkers in an early prognostic prediction and surveillance in the treatment for HCC and PVTT.
筛选与肝细胞癌（HCC）患者血清中门静脉癌栓（PVTT）相关的低分子量蛋白质生物标志物。
采集12名健康志愿者、12名无PVTT的HCC患者和12名有PVTT的HCC患者的血清样本。采用二维凝胶电泳（2-DE），其中第二维为16%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），用ImageMaster软件分析3组血清蛋白图像。差异蛋白点通过基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF MS/MS）进一步鉴定。
与使用12.5% SDS-PAGE凝胶的结果相比，16% SDS-PAGE凝胶中显示出更多的蛋白条带（3×10³至20×10³之间），且低分子量（MW）蛋白点（小于20×10³）清晰可见。通过组间比较在3组中发现了代表5种蛋白质的15个差异蛋白点，随后对其进行了鉴定。与健康组相比，载脂蛋白A-I、脂蛋白CIII、转甲状腺素蛋白和DNA拓扑异构酶II在HCC组中均下调，而触珠蛋白-2过表达。PVTT组中所有5种蛋白质的下降幅度均大于非PVTT组。
低分子量血清蛋白的表达在HCC发病初期和进展期明显变化，差异表达的低分子量蛋白可能是HCC和PVTT治疗中早期预后预测和监测的潜在生物标志物。
