在氨基酸类似物存在的情况下，肝癌细胞中合成的磷酸烯醇丙酮酸羧激酶（三磷酸鸟苷）的特性。
Properties of phosphoenolpyruvate carboxykinase (guanosine triphosphate) synthesized in hepatoma cells in the presence of amino acid analogues.
作者信息
Knowles S E, Gunn J M, Reshef L, Hanson R W, Ballard F J
出版信息
Biochem J. 1975 Mar;146(3):585-93. doi: 10.1042/bj1460585.
Abstract
Phosphoenolpyruvate carboxykinase (GTP) was induced by a combination of dibutyryl cyclic AMP, theophyline and dexamethasone in Reuber H35 hepatoma cells under conditions where an amino acid in the medium was replaced by an appropriate analogue. 2. With canavanine replacing arginine or with 5-fluorotryptophan or 6-fluorotryptophan replacing tryptophan the induced enzyme had a lower catalytic activity-relative to antibody reactivity. 3. These aberrant enzyme molecules were heat-labile in vitro. 4. Measurements of enzyme degradation in vivo indicated that the canavanine-containing enzyme and the 6-fluorotryptophan-containing enzyme were degraded more rapidly than the enzyme containing all natural amino acids.
摘要
在培养基中的一种氨基酸被适当类似物取代的条件下，磷酸烯醇丙酮酸羧激酶（GTP）在Reuber H35肝癌细胞中由二丁酰环磷酸腺苷、茶碱和地塞米松联合诱导产生。2. 用刀豆氨酸取代精氨酸，或用5-氟色氨酸或6-氟色氨酸取代色氨酸时，相对于抗体反应性，诱导产生的酶具有较低的催化活性。3. 这些异常的酶分子在体外对热不稳定。4. 体内酶降解的测量表明，含刀豆氨酸的酶和含6-氟色氨酸的酶比含所有天然氨基酸的酶降解得更快。
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