Khalsa-Moyers Gurusahai, McDonald William Hayes
Genome Science and Technology, University of Tennessee, Knoxville, USA.
Brief Funct Genomic Proteomic. 2006 Jun;5(2):98-111. doi: 10.1093/bfgp/ell023. Epub 2006 May 18.
State-of-the-art proteomics workflows involve multiple interdependent steps: sample preparation, protein-peptide separation, mass spectrometry and data analysis. While improvements in any of these steps can increase the depth and breadth of analysis, advances in mass spectrometry have catalysed many of the most important developments. We discuss common classes of mass analysers and how these analysers are put together to produce some of the most popular mass spectrometry platforms. The capabilities of these platforms determine how they can be used in a variety of common proteomic strategies and, in turn, what types of biological questions can be addressed. Moving forward, powerful new hybrid mass spectrometers and application of emerging types of tandem mass spectrometry promise that our ability to analyse complex mixtures of proteins will continue to advance.
样品制备、蛋白质-肽分离、质谱分析和数据分析。虽然这些步骤中的任何一个取得进展都能增加分析的深度和广度，但质谱分析的进步推动了许多最重要的进展。我们将讨论常见类型的质量分析器，以及这些分析器如何组合在一起形成一些最受欢迎的质谱平台。这些平台的功能决定了它们如何应用于各种常见的蛋白质组学策略，进而决定了可以解决哪些类型的生物学问题。展望未来，功能强大的新型混合质谱仪以及新兴串联质谱技术的应用有望使我们分析复杂蛋白质混合物的能力持续提升。
