Guth B E, Giraldi R, Gomes T A, Marques L R
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brazil.
Can J Microbiol. 1994 May;40(5):341-4. doi: 10.1139/m94-056.
A total of 108 Escherichia coli strains characterized as enteropathogenic (EPEC) by serotyping and the presence of EPEC adherence factor (EAF) sequences were examined for cytotoxin production by cell line assays and colony hybridization with Shiga-like toxin (SLT) probes. Cytolethal distending toxin (CLDT) production was found in three (2.8%) strains belonging to serotype O86:H34, while one O111ab:NM strain hybridized with a SLT-II probe but did not express any cytotoxic activity. All four strains showed localized adherence to HeLa cells and hybridized to an E. coli attaching-effacing gene (eae) probe. The CLDT-producing strains had multiple plasmids and some were present in all strains, including a plasmid of approximately 54 MDa that hybridized with the EAF probe.
通过血清分型和肠致病性大肠杆菌（EPEC）黏附因子（EAF）序列的存在情况鉴定为肠致病性的总共108株大肠杆菌，采用细胞系检测法和与志贺样毒素（SLT）探针进行菌落杂交来检测其细胞毒素产生情况。在属于血清型O86:H34的三株（2.8%）菌株中发现了细胞致死性膨胀毒素（CLDT）产生，而一株O111ab:NM菌株与SLT-II探针杂交，但未表现出任何细胞毒性活性。所有四株菌株均显示对HeLa细胞的局部黏附，并与大肠杆菌紧密黏附-抹平基因（eae）探针杂交。产生CLDT的菌株具有多个质粒，有些质粒存在于所有菌株中，包括一个与EAF探针杂交的约54 MDa的质粒。
