Wiebert Pernilla, Sanchez-Crespo Alejandro, Falk Rolf, Philipson Klas, Lundin Anders, Larsson Stig, Möller Winfried, Kreyling Wolfgang G, Svartengren Magnus
Department of Nuclear Medicine, Karolinska Hospital, Stockholm, Sweden.
Inhal Toxicol. 2006 Sep;18(10):741-7. doi: 10.1080/08958370600748455.
Human pulmonary retention of 35 nm 99mTc-labeled carbonaceous particles, produced with a modified Technegas generator, was followed for 24 h using a gamma camera imaging technique. Nine healthy subjects and four asthmatics inhaled the test particles. Particle labeling stability was tested in vitro during 48 h. We also measured in vivo leaching in blood and in urine for 24 h. One additional subject was exposed to particles with unstable labeling. There were no significant differences between healthy and asthmatic subjects in any of the parameters studied. Particle retention after 24 h was 102% (SD +/- 4.7). Cumulative in vitro leaching of 99mTc activity from the particles was 1.7% (+/-1.1) after 24 h. In blood samples, 80 min after exposure, 1.1% (+/- 0.4) of initially deposited activity was detected and 91% of the activity was not bound to particles. In urine sampled during the first 24 h after exposure, 3.6% (+/- 0.9) of lung deposited activity was detected. Lung retention was 30% after 1 h in the subject exposed to the leaching aerosol (n = 1). Thirty-one percent of the deposited activity was detected in the blood after 80 min and 80% was not bound to particles. Fifty percent of the activity was excreted with urine within 24 h. On gamma camera images the activity visibly translocated from lungs to thyroid and gastrointestinal tract. In conclusion, over a 24-h period there was no significant translocation of inhaled 35-nm particles to the systemic circulation.
采用伽马相机成像技术，对使用改良型锝气体发生器产生的35纳米99mTc标记碳质颗粒在人体肺部的滞留情况进行了24小时跟踪。9名健康受试者和4名哮喘患者吸入了测试颗粒。在体外对颗粒标记稳定性进行了48小时测试。我们还测量了24小时内血液和尿液中的体内浸出情况。另外一名受试者吸入了标记不稳定的颗粒。在研究的任何参数方面，健康受试者和哮喘患者之间均无显著差异。24小时后颗粒滞留率为102%（标准差±4.7）。24小时后颗粒中99mTc活性的体外累积浸出率为1.7%（±1.1）。在暴露后80分钟采集的血样中，检测到最初沉积活性的1.1%（±0.4），且91%的活性未与颗粒结合。在暴露后最初24小时采集的尿样中，检测到肺部沉积活性的3.6%（±0.9）。暴露于浸出气雾剂的受试者1小时后肺部滞留率为30%（n = 1）。80分钟后在血液中检测到31%的沉积活性，且80%未与颗粒结合。50%的活性在24小时内随尿液排出。在伽马相机图像上，活性明显从肺部转移至甲状腺和胃肠道。总之，在24小时内，吸入的35纳米颗粒向体循环的转移不显著。
