经体外转录RNA直接接种后从兔体中回收传染性兔出血症病毒
Recovery of infectious rabbit hemorrhagic disease virus from rabbits after direct inoculation with in vitro-transcribed RNA.
作者信息
Liu Guangqing, Zhang Yuying, Ni Zheng, Yun Tao, Sheng Zutian, Liang Huali, Hua Jionggang, Li Shuangmao, Du Qingyun, Chen Jianping
机构信息
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People's Republic of China.
出版信息
J Virol. 2006 Jul;80(13):6597-602. doi: 10.1128/JVI.02078-05.
Abstract
We report the first full-length infectious clone of strain JX/CHA/97 of rabbit hemorrhagic disease virus (RHDV). The transcripts from the full-length cDNA clones were infectious when they were directly injected into rabbits. The sequence of the virus recovered from the rabbits was identical to that of the injected RNA transcripts. The cDNA clone was engineered to contain one silent nucleotide change to create an EcoRV site (A to T at nucleotide 2908). The genetic marker was retained in the recovered progeny virus. The transfection of RNA transcripts into RK-13 cells resulted in the synthesis of viral antigens, indicating that the cDNA clones were replication competent. This stable infectious molecular clone should be an important tool for developing a better understanding of the molecular biology and pathogenesis of RHDV.
摘要
我们报告了兔出血症病毒（RHDV）JX/CHA/97株的首个全长感染性克隆。全长cDNA克隆的转录本直接注射到兔子体内时具有感染性。从兔子体内回收的病毒序列与注射的RNA转录本序列相同。该cDNA克隆经设计含有一个沉默核苷酸变化，以产生一个EcoRV位点（核苷酸2908处的A变为T）。该遗传标记保留在回收的子代病毒中。将RNA转录本转染到RK-13细胞中导致病毒抗原的合成，表明cDNA克隆具有复制能力。这个稳定的感染性分子克隆应该是深入了解RHDV分子生物学和发病机制的重要工具。
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