Shimizu K, Takenaka O
Department of Morphology, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Biochem Genet. 1991 Apr;29(3-4):177-87. doi: 10.1007/BF02401811.
DNA polymorphisms in the psi beta 1-beta-globin gene region in nine Asian macaques (Macaca fuscata, M. mulatta, M. nemestrina, M. cyclopis, M. fascicularis, M. arctoides, M. radiata, M. maura, and M. assamensis) were examined using several restriction endonucleasese and the human psi beta 1, delta IVS2, and beta IVS2 probes. The BamHI site 3' to the beta-globin gene was polymorphic in M. fuscata and M. mulatta, while the HincII site and the EcoRI site in the psi beta 1-globin gene region was highly polymorphic in M. fuscata and M. mulatta, respectively. These polymorphic sites also seem to be present in other Asian macaques. The present study of the polymorphism at the BamHI site 3' to the beta-globin gene in Asian macaques supports, at the nuclear DNA level, the idea that the fascicularis group including M. fuscata, M. mulatta, M. cyclopis, and M. fascicularis is different from other Asian macaque groups.
利用几种限制性内切酶以及人ψβ1、δIVS2和βIVS2探针，检测了9种亚洲猕猴（日本猕猴、恒河猴、豚尾猕猴、台湾猕猴、食蟹猕猴、熊猴、白眉猕猴、毛耳猕猴和阿萨姆猕猴）ψβ1 - β珠蛋白基因区域的DNA多态性。β珠蛋白基因3'端的BamHI位点在日本猕猴和恒河猴中具有多态性，而ψβ1 - 珠蛋白基因区域的HincII位点和EcoRI位点在日本猕猴和恒河猴中分别具有高度多态性。这些多态性位点似乎在其他亚洲猕猴中也存在。目前对亚洲猕猴β珠蛋白基因3'端BamHI位点多态性的研究在核DNA水平上支持了这样一种观点，即包括日本猕猴、恒河猴、台湾猕猴和食蟹猕猴在内的食蟹猴类群与其他亚洲猕猴类群不同。
