Büch U, Altmayer P, Isenberg J C, Büch H P
Clinic of Anesthesiology and Intensive Care Medicine, University of the Saarland, Homburg, Fed. Rep. of Germany.
Arzneimittelforschung. 1991 Apr;41(4):363-6.
In rats anesthetized with halothane (CAS 151-67-7) (1.5 vol%) and rats without any further treatment (control) the early distribution phase of thiopental (CAS 76-75-5) (i.v. 30 mg/kg) was studied. In serum and 8 tissues thiopental concentration (T) was determined using ultraviolet detection at 305 nm after extraction and TLC. In rats anesthetized with halothane, T in serum was significantly higher during the 30 min following the thiopental injection (at least +27% and maximally +51%) as compared to the control (same dose), and several pharmacokinetic parameters (e.g. central volume of distribution) were found to be changed thereby; furthermore, at 3, 10 and 30 min T was significantly increased in liver, brain, heart, lung and spleen; in kidney and skeletal muscle a rise of T was also seen, however, it occurred later (after 10 and 30 min). T in fat tissue increased time-dependently; a T-difference in adipose tissue between both groups was not observed. Thiopental was "trapped" during the early distribution phase to a considerable extent in the vessel-rich tissues of rats simultaneously anesthetized with halothane; this pharmacokinetic interaction might be explained hemodynamically: in many tissues regional blood flow is reduced by halothane; thereby a delayed "washout" of thiopental from the vessel-rich tissues could take place and redistribution would be delayed; additional factors as e.g. an increased binding of thiopental at tissue proteins could also play a role. An unusually high T was found at least temporarily in myocardial tissue due to this interaction between the two anesthetics.
在使用氟烷（CAS 151 - 67 - 7）（1.5体积%）麻醉的大鼠和未作任何进一步处理的大鼠（对照）中，研究了硫喷妥钠（CAS 76 - 75 - 5）（静脉注射30毫克/千克）的早期分布阶段。在血清和8种组织中，提取并经薄层色谱法分离后，于305纳米处采用紫外线检测法测定硫喷妥钠浓度（T）。与对照（相同剂量）相比，在使用氟烷麻醉的大鼠中，硫喷妥钠注射后30分钟内血清中的T显著升高（至少升高27%，最高升高51%），并发现一些药代动力学参数（如中央分布容积）因此发生改变；此外，在3、10和30分钟时，肝脏、大脑、心脏、肺和脾脏中的T显著升高；在肾脏和骨骼肌中也观察到T升高，不过出现得较晚（分别在10分钟和30分钟后）。脂肪组织中的T随时间增加；未观察到两组间脂肪组织中的T存在差异。在同时使用氟烷麻醉的大鼠的富血管组织中，硫喷妥钠在早期分布阶段在很大程度上被“滞留”；这种药代动力学相互作用可能从血流动力学角度得到解释：在许多组织中，氟烷会使局部血流减少；由此，硫喷妥钠从富血管组织中的“清除”会延迟，再分布也会延迟；其他因素如硫喷妥钠与组织蛋白结合增加也可能起作用。由于这两种麻醉剂之间的这种相互作用，在心肌组织中至少暂时发现了异常高的T。
