Su Jung-Jeng, Yeh Kuang-Sheng, Tseng Pin-Wei
Division of Applied Biology, Animal Technology Institute Taiwan, Ding-Pu, Chunan, Miaoli, Taiwan, Republic of China.
Curr Microbiol. 2006 Jul;53(1):77-81. doi: 10.1007/s00284-006-0021-x. Epub 2006 Jun 9.
A high concentration of NH(4) (+) in piggery wastewater is major problem in Taiwan. Therefore, in our study, we isolated native heterotrophic nitrifiers for piggery wastewater treatment. Heterotrophic nitrifier AS-1 was isolated and characterized from the activated sludge of a piggery wastewater system. Sets of triplicate crimp-sealed serum bottles were used to demonstrate the heterotrophic nitrifying capability of strain AS-1 in an incubator at 30 degrees C. All serum bottles contained 80 mL medium, and the remainder of the bottle headspace was filled with pure oxygen. The experimental results showed that 2.5 +/- 0.2 mmol L(-1) NH(4) (+) was removed by 58 hours, and, eventually, 1.5 +/- 0.5 mmol L(-1) N(2) and 0.2 +/- 0.0 mmol L(-1) N(2)O were produced. The removal rate of NH(4) (+) by the strain AS-1 was 1.75 mmol NH(4) (+) g cell(-1) h(-1). This strain was then identified as Pseudomonas alcaligenes (97% identity) by sequencing its 16S rDNA and comparing it with other microorganisms. Thus, strain AS-1 displays high promise for future application for in situ NH(4) (+) removal from piggery wastewater.
养猪废水中高浓度的NH(4)(+)是台湾地区的一个主要问题。因此，在我们的研究中，我们分离出了用于处理养猪废水的本地异养硝化菌。异养硝化菌AS-1是从一个养猪废水系统的活性污泥中分离并鉴定出来的。使用了三组一式三份的压接密封血清瓶，在30摄氏度的培养箱中展示菌株AS-1的异养硝化能力。所有血清瓶都含有80 mL培养基，瓶顶空间的其余部分充满纯氧。实验结果表明，58小时内去除了2.5±0.2 mmol L(-1) NH(4)(+)，最终产生了1.5±0.5 mmol L(-1) N(2)和0.2±0.0 mmol L(-1) N(2)O。菌株AS-1对NH(4)(+)的去除率为1.75 mmol NH(4)(+) g细胞(-1) h(-1)。通过对其16S rDNA进行测序并与其他微生物进行比较，该菌株随后被鉴定为产碱假单胞菌（同一性为97%）。因此，菌株AS-1在未来用于原位去除养猪废水中的NH(4)(+)方面具有很高的应用前景。
