Key Laboratory for Urban Habitat Environmental Science and Technology, School of Environment and Energy, Peking University Shenzhen Graduate School, 518055, Shenzhen, China.
J Ind Microbiol Biotechnol. 2011 Sep;38(9):1305-10. doi: 10.1007/s10295-010-0911-6. Epub 2010 Nov 27.
Three novel strains capable of heterotrophic nitrification-aerobic denitrification were isolated from the landfill leachate treatment system. Based on their phenotypic and phylogenetic characteristics, the isolates were identified as Agrobacterium sp. LAD9, Achromobacter sp. GAD3 and Comamonas sp. GAD4, respectively. Batch tests were carried out to evaluate the growth and the ammonia removal patterns. The maximum growth rates as determined from the growth curve were 0.286, 0.228, and 0.433 h(-1) for LAD9, GAD3 and GAD4, respectively. The maximum aerobic nitrification-denitrification rate was achieved by the strain GAD4 of 0.381 mmol/l h, followed by LAD9 of 0.374 mmol/l h and GAD3 of 0.346 mmol/l h. Moreover, hydroxylamine oxidase and periplasmic nitrate reductase were successfully expressed in all the isolates. The relationship between the enzyme activities and the aerobic nitrification-denitrification rates revealed that hydroxylamine oxidation may be the rate-limiting step in the heterotrophic nitrification-aerobic denitrification process. The study results are of great significance to the wastewater treatment systems where simultaneous removal of carbon and nitrogen is desired.
从垃圾渗滤液处理系统中分离到三株能够进行异养硝化-好氧反硝化的新型菌株。根据其表型和系统发育特征，将这些分离株分别鉴定为根瘤农杆菌 LAD9、无色杆菌 GAD3 和食酸丛毛单胞菌 GAD4。进行了批处理试验以评估生长和氨去除模式。从生长曲线确定的最大生长速率分别为 LAD9、GAD3 和 GAD4 的 0.286、0.228 和 0.433 h(-1)。最大好氧硝化-反硝化速率由 GAD4 菌株达到 0.381 mmol/l h，其次是 LAD9 菌株的 0.374 mmol/l h 和 GAD3 菌株的 0.346 mmol/l h。此外，所有分离株中均成功表达了羟胺氧化酶和周质硝酸盐还原酶。酶活性与好氧硝化-反硝化速率之间的关系表明，羟胺氧化可能是异养硝化-好氧反硝化过程中的限速步骤。该研究结果对同时去除碳和氮的废水处理系统具有重要意义。
