Qiu F F, Nie Q H, Luo C L, Zhang D X, Lin S M, Zhang X Q
Department of Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong.
Poult Sci. 2006 Jun;85(6):980-5. doi: 10.1093/ps/85.6.980.
Growth rate, body composition, and fat deposition are important traits in chickens. Insulin plays important roles in hepatic cells, muscle cells, and adipose tissue cells. The purpose of the present study was to analyze association of the insulin (INS) gene with chicken growth and body composition traits. Using a F2 design resource population constructed with the crossing of Chinese native Xinghua chickens and White Recessive Rock chickens, the association of 4 single nucleotide polymorphisms (SNP; A+428G, C+1549T, T+3737C, and A+3971G) of INS gene with 13 growth and body composition traits was studied. The T+3737C genotypes were significantly associated with small intestine length (P = 0.0002), and the A+3971G genotypes were significantly associated with early growth (hatch weight and BW at 28 d of age) (P < 0.0001), breast angle (P = 0.0002), and small intestine length (P < 0.0001). None of the 4 SNP was significantly associated with abdominal fat pad weight (P > 0.05). The haplotypes based on the 4 SNP were also significantly associated with early growth (hatch weight and BW at 28 d of age; P < 0.0001) and breast angle (P < 0.0001) but not with small intestine length (P = 0.0505). These results suggested that variation of the insulin gene was significantly associated with chicken early growth but not with fat deposition. In addition, the data from the present study supported the inference that both the one-SNP-at-a-time and the haplotype-based approaches have their own advantages and disadvantages when association analysis of one SNP and haplotypes with chicken complex traits was conducted.
生长速度、身体组成和脂肪沉积是鸡的重要性状。胰岛素在肝细胞、肌肉细胞和脂肪组织细胞中发挥重要作用。本研究的目的是分析胰岛素（INS）基因与鸡生长和身体组成性状的关联。利用中国本土杏花鸡与隐性白洛克鸡杂交构建的F2设计资源群体，研究了INS基因的4个单核苷酸多态性（SNP；A+428G、C+1549T、T+3737C和A+3971G）与13个生长和身体组成性状的关联。T+3737C基因型与小肠长度显著相关（P = 0.0002），A+3971G基因型与早期生长（出雏重和28日龄体重）（P < 0.0001）、胸角（P = 0.0002）和小肠长度（P < 0.0001）显著相关。4个SNP均与腹脂垫重量无显著关联（P > 0.05）。基于这4个SNP的单倍型也与早期生长（出雏重和28日龄体重；P < 0.0001）和胸角（P < 0.0001）显著相关，但与小肠长度无关（P = 0.0505）。这些结果表明，胰岛素基因的变异与鸡的早期生长显著相关，但与脂肪沉积无关。此外，本研究的数据支持了这样的推断：在对一个SNP和单倍型与鸡复杂性状进行关联分析时，一次一个SNP的方法和基于单倍型的方法都有各自的优缺点。
