College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, P. R. China.
J Appl Genet. 2010;51(1):59-65. doi: 10.1007/BF03195711.
Visfatin is a peptide that is predominantly expressed in visceral adipose tissue and is hypothesized to be related to obesity and insulin resistance. In this study, a novel silent single-nucleotide polymorphism (SNP) was found in exon 7 of the chicken visfatin gene (also known as PBEF1) by single-stranded conformation polymorphism (SSCP) and DNA sequencing. In total, 836 chickens forming an F2 resource population of Gushi chicken crossed with Anka broiler were genotyped by XbaI forced RFLP, and the associations of this polymorphism with chicken growth, carcass characteristics, and meat quality were analyzed. Significant associations were found between the polymorphism and 4-week body weight (BW4), 6-week body weight (BW6), 4-week body slanting length (BSL4), fat bandwidth (FBW), breast muscle water loss rate (BWLR) and breast muscle fiber density (BFD) (P < 0.05), as well as 4-week breastbone length (BBL4) (P < 0.01). These observations suggested that the polymorphism in exon7 of the visfatin gene had significant effects on the early growth traits of chicken.
内脂素是一种主要在内脏脂肪组织中表达的肽，与肥胖和胰岛素抵抗有关。本研究通过单链构象多态性（SSCP）和 DNA 测序发现鸡内脂素基因（也称为 PBEF1）外显子 7 中有一个新的沉默单核苷酸多态性（SNP）。总共对 836 只形成固始鸡与安卡肉鸡杂交 F2 资源群体的鸡进行 XbaI 强制 RFLP 基因分型，分析该多态性与鸡生长、胴体特征和肉质的关系。该多态性与 4 周体重（BW4）、6 周体重（BW6）、4 周体斜长（BSL4）、脂肪带宽（FBW）、胸肌失水率（BWLR）和胸肌纤维密度（BFD）显著相关（P < 0.05），以及 4 周胸骨长度（BBL4）显著相关（P < 0.01）。这些观察结果表明，内脂素基因外显子 7 中的多态性对鸡的早期生长性状有显著影响。
