Kim Mi-Hyun, Park Seong-Cheol, Kim Jin-Young, Lee Sun Young, Lim Hak-Tae, Cheong Hyeonsook, Hahm Kyung-Soo, Park Yoonkyung
Research Center for Proteineous Materials, Chosun University, Kwangju 501-759, Republic of Korea.
Biochem Biophys Res Commun. 2006 Aug 4;346(3):681-6. doi: 10.1016/j.bbrc.2006.05.186. Epub 2006 Jun 9.
Potide-G, a small (5578.9 Da) antimicrobial peptide, was isolated from potato tubers (Solanum tuberosum L. cv. Golden Valley) through extraction of the water-soluble fraction, dialysis, ultrafiltration and DEAE-cellulose and C18 reverse-phase high performance liquid chromatography. This antimicrobial peptide was heat-stable and almost completely suppressed the proteolytic activity of trypsin, chymotrypsin and papain, with no hemolytic activity. In addition, potide-G potently inhibited growth of a variety of bacterial (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Clavibacter michiganense subsp. michiganinse) and fungal (Candida albicans and Rhizoctonia solani) strains. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry revealed that the N-terminal sequence (residues from 1 to 11) of the protein is identical to that of potato proteinase inhibitor, a member of the Kunitz superfamily. And like other members of this class of protease inhibitor, potide-G may have a number of beneficial and therapeutic uses.
Potide-G是一种小分子（5578.9道尔顿）抗菌肽，通过提取水溶性部分、透析、超滤以及DEAE-纤维素和C18反相高效液相色谱法从马铃薯块茎（马铃薯品种金谷）中分离得到。这种抗菌肽具有热稳定性，几乎能完全抑制胰蛋白酶、糜蛋白酶和木瓜蛋白酶的蛋白水解活性，且无溶血活性。此外，Potide-G能有效抑制多种细菌（金黄色葡萄球菌、单核细胞增生李斯特菌、大肠杆菌和密执安棒杆菌密执安亚种）和真菌（白色念珠菌和立枯丝核菌）菌株的生长。基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱分析表明，该蛋白的N端序列（第1至11位氨基酸残基）与马铃薯蛋白酶抑制剂相同，马铃薯蛋白酶抑制剂是库尼茨超家族的成员之一。与这类蛋白酶抑制剂的其他成员一样，Potide-G可能具有多种有益的治疗用途。
