Brisson-Noel A, Aznar C, Chureau C, Nguyen S, Pierre C, Bartoli M, Bonete R, Pialoux G, Gicquel B, Garrigue G
Diagnostics Pasteur, Marnes-la-Coquette, France.
Lancet. 1991 Aug 10;338(8763):364-6. doi: 10.1016/0140-6736(91)90492-8.
Various polymerase chain reaction (PCR) assays have been devised for the rapid identification of mycobacteria in clinical specimens. To assess the value of such assays in routine laboratory work the results obtained by PCR were compared with those obtained by standard microbiological methods for 514 specimens collected for investigation of mycobacterial infection. Specimens were tested for the presence of Mycobacterium tuberculosis complex and atypical mycobacteria in two assays, one based on amplification of the 65 kDa gene and the other on the IS6110 insertion sequence. For the 489 samples that did not contain inhibitors of the amplification reaction PCR findings correlated well with bacteriological and/or clinical data in 476 (97.4%). 6 PCR results turned out to be false negatives, 3 to be false positives and 4 to be mis-identification of strains. Pre-treatment of samples with guanidium thiocyanate reduced the proportion of false-negative results and of samples that contained inhibitors. This study confirms the potential of DNA amplification for early diagnosis of mycobacterial infections.
已经设计出各种聚合酶链反应（PCR）检测方法，用于快速鉴定临床标本中的分枝杆菌。为了评估此类检测方法在常规实验室工作中的价值，将PCR获得的结果与通过标准微生物学方法对514份为调查分枝杆菌感染而采集的标本所获得的结果进行了比较。在两种检测方法中对标本进行结核分枝杆菌复合群和非典型分枝杆菌检测，一种基于65 kDa基因的扩增，另一种基于IS6110插入序列。对于489份不含扩增反应抑制剂的样本，PCR结果与细菌学和/或临床数据在476份样本中相关性良好（97.4%）。6份PCR结果为假阴性，3份为假阳性，4份为菌株误鉴定。用硫氰酸胍对样本进行预处理可降低假阴性结果的比例以及含有抑制剂的样本比例。本研究证实了DNA扩增在分枝杆菌感染早期诊断中的潜力。
