van Geel Nanny, Ongenae Katia, Vander Haeghen Yves, Vervaet Chris, Naeyaert Jean Marie
Department of Dermatology, Ghent University Hospital, Ghent, Belgium.
Dermatology. 2006;213(1):23-9. doi: 10.1159/000092833.
Noncultured epidermal cell transplantation in vitiligo permits the coverage of relatively large areas without culturing cells.
To investigate the effectiveness of noncultured epidermal cell transplantation in treating stabilized vitiligo using objective and subjective evaluation methods.
Noncultured autologous melanocytes and keratinocytes were grafted in a hyaluronic-acid-enriched suspension on superficially laser-abraded vitiligo lesions in 40 patients with refractory stable vitiligo (30 with generalized and 10 with localized vitiligo). The repigmentation was evaluated 3-12 months after grafting using a digital image analysis system. Furthermore the treatment was evaluated from the patients' point of view with the DLQI (Dermatology Life Quality Index) and a 'global assessment'.
The mean percentage of repigmentation, evaluated at the last follow-up visit, was 72% (median 84%), and a repigmentation of >or=70% was observed in 62% of patients. The best results were achieved in the neck and the presternal region. A subjective evaluation was performed in half of the subjects. The mean DLQI score at inclusion (6.95, SD = 6.68, n = 20) was significantly decreased after treatment (p = 0.013, mean 3.85, SD = 4.13, n = 20). The patients were satisfied with the achieved result, found it worthwhile to undergo the treatment and would choose it again.
According to both subjective and objective evaluation methods, noncultured epidermal cell transplantation is promising in patients with stable vitiligo.
白癜风的非培养表皮细胞移植可在不培养细胞的情况下覆盖相对较大的面积。
采用客观和主观评估方法，研究非培养表皮细胞移植治疗稳定期白癜风的有效性。
将非培养的自体黑素细胞和角质形成细胞以富含透明质酸的悬液移植到40例难治性稳定期白癜风患者（30例泛发型、10例局限型白癜风）经浅表激光磨皮的白癜风皮损上。移植后3至12个月，使用数字图像分析系统评估色素恢复情况。此外，从患者角度采用皮肤病生活质量指数（DLQI）和“整体评估”对治疗进行评价。
在最后一次随访时评估的色素恢复平均百分比为72%（中位数84%），62%的患者色素恢复≥70%。颈部和胸骨前区域效果最佳。对一半受试者进行了主观评估。纳入时的平均DLQI评分（6.95，标准差=6.68，n=20）在治疗后显著降低（p=0.013，平均3.85，标准差=4.13，n=20）。患者对取得的结果满意，认为接受该治疗值得且会再次选择。
根据主观和客观评估方法，非培养表皮细胞移植对稳定期白癜风患者有前景。
