Wang Yong-Jun, Shi Qi, Lu W W, Cheung K C M, Darowish Michael, Li Tian-Fang, Dong Yu-Feng, Zhou Chong-Jian, Zhou Quan, Hu Zhi-Jun, Liu Mei, Bian Qin, Li Chen-Guang, Luk K D K, Leong J C Y
Institute of Spine, ShangHai University of Traditional Chinese Medicine. Shanghai, China.
Spine (Phila Pa 1976). 2006 Jun 15;31(14):1532-8. doi: 10.1097/01.brs.0000222019.84095.23.
Establishment of a novel in vivo animal model of cervical spondylosis.
To investigate apoptotic, degenerative, and inflammatory changes occurring in the cervical intervertebral discs of rats.
Cervical degeneration occurs as the result of imbalance of both static and dynamic spinal stabilizers. The disc degeneration that occurs is characterized by increased local inflammation and increased apoptosis of intervertebral disc cells.
By excising the paraspinal musculature and posterior cervical spinal ligaments of rats, both static and dynamic cervical stabilizers were disrupted. The resultant biomechanical imbalance resulted in biochemical and histologic changes, which were characterized by light microscopy, electron microscopy, immunostaining, enzyme-linked immunosorbent assay, polymerase chain reaction, and in situ hybridization.
Histologic analysis showed characteristic degenerative changes of the intervertebral discs and vertebral endplates following surgery. Ultrastructural examination revealed apoptotic changes, which were verified by immunostaining. Instability also resulted in significant up-regulation of inflammatory factors, as shown by enzyme-linked immunosorbent assay, polymerase chain reaction, and in situ hybridization.
By creating static and dynamic posterior instability of the cervical spine, this novel model of cervical spondylosis results in rapid intervertebral disc degeneration characterized by increased apoptosis and local inflammation, such as that seen clinically.
建立一种新型的颈椎病体内动物模型。
研究大鼠颈椎间盘发生的凋亡、退变及炎症变化。
颈椎退变是由脊柱静态和动态稳定器失衡所致。发生的椎间盘退变以局部炎症增加和椎间盘细胞凋亡增加为特征。
通过切除大鼠脊柱旁肌肉组织和颈后部脊柱韧带，破坏颈椎的静态和动态稳定器。由此产生的生物力学失衡导致生化和组织学变化，通过光学显微镜、电子显微镜、免疫染色、酶联免疫吸附测定、聚合酶链反应和原位杂交进行表征。
组织学分析显示术后椎间盘和椎体终板有特征性退变变化。超微结构检查显示有凋亡变化，免疫染色证实了这一点。如酶联免疫吸附测定、聚合酶链反应和原位杂交所示，不稳定还导致炎症因子显著上调。
通过造成颈椎的静态和动态后柱不稳定，这种新型颈椎病模型导致椎间盘快速退变，其特征为凋亡增加和局部炎症，与临床所见相似。
