Mascagni P, Tonolo M, Ball H, Lim M, Ellis R J, Coates A
Department of Pharmaceutical Chemistry, School of Pharmacy, London, UK.
FEBS Lett. 1991 Jul 29;286(1-2):201-3. doi: 10.1016/0014-5793(91)80973-7.
Molecular chaperones are required for the correct folding and assembly of certain other polypeptides. It is not known whether molecular chaperones themselves require other chaperones to become functional. A 97-amino acid chaperone, the chaperonin 10 protein was chemically synthesised so that during synthesis and purification there was no contact of the chaperone with any other protein. The purified, synthetic chaperonin 10 protein formed oligomeric structures spontaneously and was biologically active as a chaperonin. This is the first description of a chemically synthesised chaperonin, and suggests that no other chaperones are required for correct folding, polymerisation and biological activity of this chaperone.
分子伴侣是某些其他多肽正确折叠和组装所必需的。目前尚不清楚分子伴侣自身是否需要其他伴侣才能发挥功能。一种由97个氨基酸组成的伴侣蛋白，伴侣素10蛋白被化学合成，使得在合成和纯化过程中该伴侣蛋白不与任何其他蛋白质接触。纯化后的合成伴侣素10蛋白能自发形成寡聚结构，并且作为伴侣素具有生物活性。这是对化学合成伴侣素的首次描述，表明该伴侣蛋白的正确折叠、聚合和生物活性不需要其他伴侣蛋白。
