Lipner Ettie M, Dembele Noumouza, Souleymane Sanou, Alley William Soumbey, Prevots D Rebecca, Toe Laurent, Boatin Boayke, Weil Gary J, Nutman Thomas B
Office of Global Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
J Infect Dis. 2006 Jul 15;194(2):216-21. doi: 10.1086/505081. Epub 2006 Jun 14.
A previously developed, specific, rapid-format immunochromatographic card test that detects immunoglobulin G4 to the recombinant Onchocerca volvulus antigen Ov-16 was modified to detect antibodies in whole blood.
Ov-16 card test results were assessed in 1511 subjects > or =2 years of age in 7 West African villages with varying histories of onchocerciasis control measures.
In villages in which control measures had been implemented, anti-Ov-16 antibody prevalence rates ranged from 5.2% to 65.1%. Antibody prevalence rates were close to zero among subjects born after effective control measures had been implemented. In 2 villages without a history of control measures where onchocerciasis was endemic, microfilariae (MF) prevalence rates were 82.8% and 65.1%, and antibody prevalence rates were 73.1% and 62.1%. In these 2 villages, the sensitivity of the Ov-16 card test was 81.1% and 76.5%, the specificity was 100%, and the positive predictive value was 91.8% and 80.5%. MF and antibody prevalence rates were correlated (Spearman's r=0.815; P<.038).
The Ov-16 card test is field applicable, exhibits high sensitivity and specificity for O. volvulus infection, and has great potential as a tool for surveillance and for evaluating the success of onchocerciasis control measures.
一种先前开发的、特异性的、快速格式的免疫层析卡式检测方法，用于检测针对重组盘尾丝虫抗原Ov-16的免疫球蛋白G4，现被改良以检测全血中的抗体。
在7个西非村庄中，对1511名年龄≥2岁、盘尾丝虫病控制措施实施历史各异的受试者进行了Ov-16卡式检测结果评估。
在已实施控制措施的村庄中，抗Ov-16抗体流行率在5.2%至65.1%之间。在有效控制措施实施后出生的受试者中，抗体流行率接近于零。在2个无控制措施历史且盘尾丝虫病为地方病的村庄中，微丝蚴（MF）流行率分别为82.8%和65.1%，抗体流行率分别为73.1%和62.1%。在这2个村庄中，Ov-16卡式检测的敏感性分别为81.1%和76.5%，特异性为100%，阳性预测值分别为91.8%和80.5%。MF和抗体流行率具有相关性（Spearman's r = 0.815；P <.038）。
Ov-16卡式检测适用于现场，对盘尾丝虫感染具有高敏感性和特异性，作为监测工具以及评估盘尾丝虫病控制措施成效的工具具有巨大潜力。
