Li Yanzhong, Wang Yan, Zhang Qinghui
The Key Laboratory of Otolaryngology of Health Ministry, Shandong University, Jinan, 250012, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 Apr;20(7):315-8.
To explore the role of cell adhesion molecules (CAMs) and nitric oxide synthase (NOS) in the pathological mechanism of allergic rhinitis.
Three kinds of CAMs, which are intercellular adhesion molecule-1 (ICAM-1), vascular cellular adhesion molecule-1 (VCAM-1) and lymphocyte function antigen-1 (LFA-1), as well as three kinds of NOS, which are neuronal nitric oxide synthase (nNOS), inducible isoform of nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) were orthotopic detected by strept avidin-biotin complex (SABC) immunohistochemistry method in 40 cases of inferior nasal concha of allergic rhinitis.
There are only small number of ICAM-1, VCAM-1 and LFA-1 positive cells in the normal concha mucosa epithelial but more positive cells in allergic rhinitis. The number of positive cell of ICAM-1, LFA-1, VCAM-1 in the concha mucosa was [(14.4+/-2.2), (17.2+/-3.3), (11.5+/-2.7) per scope (x400) +/-s] respectively; in the control group the number of positive cell of ICAM-1, LFA-1, VCAM-1 in the concha mucosa was (8.7+/-1.8), (10.3+/-2.1), (6.9+/-1.8) respectively. The statistic differences were significant (t = 11.57, 10.02, 8.07 respectively; P<0.01). A certain number of nNOS positive cells were found in the concha mucosa in allergic rhinitis patients, it was mainly expressed in mucosa epithelial and submucosa glandular; a large number of iNOS positive cells were found in the concha mucosa in allergic rhinitis patients, iNOS was mainly expressed in mucosa epithelial, submucosa glandular, endothelial and submucosa inflammatory cell; the number of eNOS positive cell was few. In the normal control, few positive cells of nNOS, iNOS or eNOS could be found. Between the allergic rhinitis patients and the normal control, the number of nNOS positive cell was [(9.4+/-1.7), (4.7+/-1.3) per scope (x400)] respectively, t = 12.62 (P<0.01); the number of iNOS positive cell was (27.5+/-3.2), (4.3+/-1.7) respectively, t = 36.03 (P<0.01) the number of eNOS positive cell was (6.5+/-2.1), (6.2+/-1.9) respectively, t = 0.62 (P>0.05).
These adhesion molecules might be involved in edema, exudation and infiltration of inflammatory cells of nasal mucosa in allergic rhinitis patients. The number of nNOS, iNOS positive cell in inferior concha mucosa in allergic rhinitis patients was significant higher than in normal control. It suggests that nNOS and iNOS might be involved in the pathogenesis of allergic rhinitis.
探讨细胞黏附分子（CAMs）和一氧化氮合酶（NOS）在变应性鼻炎发病机制中的作用。
采用链霉亲和素-生物素复合物（SABC）免疫组化法对40例变应性鼻炎患者下鼻甲组织中细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）、淋巴细胞功能相关抗原-1（LFA-1）3种细胞黏附分子及神经元型一氧化氮合酶（nNOS）、诱导型一氧化氮合酶（iNOS）、内皮型一氧化氮合酶（eNOS）3种一氧化氮合酶进行原位检测。
正常鼻甲黏膜上皮中ICAM-1、VCAM-1、LFA-1阳性细胞较少，变应性鼻炎时阳性细胞增多。鼻甲黏膜中ICAM-1、LFA-1、VCAM-1阳性细胞数分别为[（14.4±2.2）、（17.2±3.3）、（11.5±2.7）个/视野（×400）±s]；对照组鼻甲黏膜中ICAM-1、LFA-1、VCAM-1阳性细胞数分别为（8.7±1.8）、（10.3±2.1）、（6.9±1.8）个。差异有统计学意义（t值分别为11.57、10.02、8.07；P<0.01）。变应性鼻炎患者鼻甲黏膜中有一定数量nNOS阳性细胞，主要表达于黏膜上皮及黏膜下腺体；有大量iNOS阳性细胞，主要表达于黏膜上皮、黏膜下腺体、内皮细胞及黏膜下炎性细胞；eNOS阳性细胞较少。正常对照组nNOS、iNOS、eNOS阳性细胞少见。变应性鼻炎患者与正常对照组相比，nNOS阳性细胞数分别为[（9.4±1.7）、（4.7±1.3）个/视野（×400）]，t=12.62（P<0.01）；iNOS阳性细胞数分别为（27.5±3.2）、（4.3±1.7）个，t=36.03（P<0.01）；eNOS阳性细胞数分别为（6.5±2.1）、（6.2±1.9）个，t=0.62（P>0.05）。
这些黏附分子可能参与变应性鼻炎患者鼻黏膜水肿、渗出及炎性细胞浸润过程。变应性鼻炎患者下鼻甲黏膜中nNOS、iNOS阳性细胞数明显高于正常对照，提示nNOS和iNOS可能参与变应性鼻炎的发病机制。
