Zhao Yu, Van Hasselt C A, Woo Kangsang, Wong Yeukoi, Leung Pingcheung
Department of Otolaryngology, Prince of Wales Hospital, Chinese University of Hongkong, Shatin, NT, Hongkong SAR, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2005 Mar;19(5):219-21.
To study the immunoreactivity and distribution of both iNOS and eNOS isoforms, and observe the effect of desloratadine on them.
The guinea pigs were sensitized and challenged followed by harvest of their nasal tissue for immunohistochemical staining. The slides images were semiquantitatively analyzed and compared with the desloratadine treated group and negative control group.
Both iNOS and eNOS were positively stained in each group. The immunoreactivity of iNOS had no significant difference between groups (P > 0.05), but eNOS had stronger immunoreactivity in the model group and the desloratadine treated group when compared with the negative control group (P < 0.01 and P < 0.05). In addition to the distribution area of iNOS, eNOS was also positive stained in the goblet cells. Desloratadine had no influence on iNOS and eNOS immunoreactivity.
eNOS might play a more important role than iNOS in regulating the NO level of the nasal tissue of the guinea pigs suffered from allergic rhinitis, and desloratadine had no involvement in regulating the expression of iNOS and eNOS.
研究诱导型一氧化氮合酶（iNOS）和内皮型一氧化氮合酶（eNOS）同工型的免疫反应性及分布，并观察地氯雷他定对其的影响。
对豚鼠进行致敏和激发，随后采集其鼻组织进行免疫组织化学染色。对载玻片图像进行半定量分析，并与地氯雷他定治疗组和阴性对照组进行比较。
各组iNOS和eNOS均呈阳性染色。iNOS的免疫反应性在各组间无显著差异（P>0.05），但与阴性对照组相比，模型组和地氯雷他定治疗组的eNOS免疫反应性更强（P<0.01和P<0.05）。除iNOS的分布区域外，杯状细胞中eNOS也呈阳性染色。地氯雷他定对iNOS和eNOS的免疫反应性无影响。
在调节变应性鼻炎豚鼠鼻组织的一氧化氮水平方面，eNOS可能比iNOS发挥更重要的作用，且地氯雷他定不参与调节iNOS和eNOS的表达。
