Yuksel Hasan, Kirmaz Cengiz, Yilmaz Ozge, Pinar Ercan, Vatansever Seda, Degirmenci Papatya Bayrak, Ozbilgin Kemal
Department of Pediatric Allergy and Pulmonology, Celal Bayar University, Manisa, Turkey.
Ann Allergy Asthma Immunol. 2008 Jan;100(1):12-6. doi: 10.1016/S1081-1206(10)60398-5.
Nitric oxide (NO) has contradictory roles in the pathophysiology of allergic inflammation in both allergic rhinitis (AR) and asthma. Small amounts of NO produced by constitutive NO synthase (NOS) is anti-inflammatory, whereas large amounts produced by inducible NOS (iNOS) are proinflammatory.
To investigate the difference in constitutive endothelial NOS (eNOS) and iNOS expression in nonallergic and allergic mucosa and the possible relation of this to the coexistence of asthma in seasonal AR.
Seventeen patients (10 women and 7 men) with seasonal AR and 9 nonallergic patients (5 women and 4 men) with nasal septum deviation were enrolled. Inferior turbinate nasal biopsy specimens were obtained in all. Levels of eNOS and iNOS expressed as immunohistochemical scores (HSCOREs) were determined immunohistochemically from the specimens.
The mean +/- SD HSCOREs for eNOS in patients with seasonal AR were not significantly different from those of the nonallergic controls (1.85 +/- 0.78 vs 1.63 +/- 0.54; P = .12). On the other hand, the mean +/- SD HSCOREs for iNOS were significantly higher in patients with seasonal AR (1.75 +/- 0.75 vs 0.71 +/- 0.6; P = .004). Furthermore, although eNOS expression was not different between seasonal AR patients with and without asthma, the mean +/- SD HSCOREs for iNOS were significantly higher in the patients with asthma (1.93 +/- 0.78 vs 1.65 +/- 0.55; P = .01).
Increased expression of iNOS might have a role in the development of allergic inflammation in upper and lower airways and in comorbidity of AR and asthma.
一氧化氮（NO）在变应性鼻炎（AR）和哮喘的变应性炎症病理生理学中具有相互矛盾的作用。组成型一氧化氮合酶（NOS）产生的少量NO具有抗炎作用，而诱导型NOS（iNOS）产生的大量NO则具有促炎作用。
研究非变应性和变应性黏膜中组成型内皮型NOS（eNOS）和iNOS表达的差异，以及其与季节性AR合并哮喘的可能关系。
纳入17例季节性AR患者（10例女性和7例男性）和9例鼻中隔偏曲的非变应性患者（5例女性和4例男性）。所有患者均获取下鼻甲鼻活检标本。通过免疫组织化学方法从标本中测定以免疫组织化学评分（HSCORE）表示的eNOS和iNOS水平。
季节性AR患者中eNOS的平均±标准差HSCORE与非变应性对照组无显著差异（1.85±0.78 vs 1.63±0.54；P = 0.12）。另一方面，季节性AR患者中iNOS的平均±标准差HSCORE显著更高（1.75±0.75 vs 0.71±0.6；P = 0.004）。此外，尽管有哮喘和无哮喘的季节性AR患者之间eNOS表达无差异，但哮喘患者中iNOS的平均±标准差HSCORE显著更高（1.93±0.78 vs 1.65±0.55；P = 0.01）。
iNOS表达增加可能在上、下气道变应性炎症的发生以及AR与哮喘的合并症中起作用。
