Oliveberg Mikael, Wolynes Peter G
Department of Biochemistry and Biophysics, Arrhenius Laboratories of Natural Sciences, Stockholm University, Stockholm, Sweden.
Q Rev Biophys. 2005 Aug;38(3):245-88. doi: 10.1017/S0033583506004185. Epub 2006 Jun 19.
We review what has been learned about the protein-folding problem from experimental kinetic studies. These studies reveal patterns of both great richness and surprising simplicity. The patterns can be interpreted in terms of proteins possessing an energy landscape which is largely, but not completely, funnel-like. Issues such as speed limitations of folding, the robustness of folding, the origin of barriers and cooperativity and the ensemble nature of transition states, intermediate and traps are assessed using the results from several experimental groups highlighting energy-landscape ideas as an interpretive framework.
我们回顾了从实验动力学研究中所学到的关于蛋白质折叠问题的知识。这些研究揭示了丰富多样且令人惊讶的简单模式。这些模式可以根据蛋白质具有的能量景观来解释，该能量景观在很大程度上但并非完全呈漏斗状。利用几个实验组的结果评估了诸如折叠速度限制、折叠的稳健性、障碍和协同性的起源以及过渡态、中间体和陷阱的集合性质等问题，突出了能量景观概念作为一种解释框架。
