Komiya Migiwa, Takeuchi Takashi, Harada Etsumori
Graduate School of Veterinary Science, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
Behav Brain Res. 2006 Sep 25;172(2):240-9. doi: 10.1016/j.bbr.2006.05.006. Epub 2006 Jun 15.
We examined the anti-stress action of the essential oils of lavender, rose, and lemon using an elevated plus-maze task (EPM), a forced swimming task (FST), and an open field task (OFT) in mice. Lemon oil had the strongest anti-stress effect in all three behavioral tasks. We further investigated a regulatory mechanism of the lemon oil by pre-treatments with agonists or antagonists to benzodiazepine, 5-HT, DA, and adrenaline receptors by the EPM and the FST. The anti-stress effect of lemon oil was significantly blocked by pre-treatment with frumazenil, benzodiazepine receptor antagonist, or apomorphine, a nonselective DA receptor agonist. In contrast, agonists or antagonists to the 5-HT receptor and the alpha-2 adrenaline receptor did not affect the anti-stress effect of lemon oil. Buspirone, DOI, and mianserine blocked the antidepressant-like effect of lemon oil in the FST, but WAY100,635 did not. These findings suggest that the antidepressant-like effect of lemon oil is closely related with the 5-HTnergic pathway, especially via 5-HT(1A) receptor. Moreover, the lemon oil significantly accelerated the metabolic turnover of DA in the hippocampus and of 5-HT in the prefrontal cortex and striatum. These results suggest that lemon oil possesses anxiolytic, antidepressant-like effects via the suppression of DA activity related to enhanced 5-HTnergic neurons.
我们使用高架十字迷宫任务（EPM）、强迫游泳任务（FST）和旷场任务（OFT），在小鼠中研究了薰衣草、玫瑰和柠檬精油的抗应激作用。在所有这三项行为任务中，柠檬精油具有最强的抗应激效果。我们通过使用EPM和FST，用苯二氮䓬、5-羟色胺、多巴胺和肾上腺素受体的激动剂或拮抗剂进行预处理，进一步研究了柠檬精油的调节机制。用氟马西尼（一种苯二氮䓬受体拮抗剂）或阿扑吗啡（一种非选择性多巴胺受体激动剂）预处理，可显著阻断柠檬精油的抗应激作用。相比之下，5-羟色胺受体和α-2肾上腺素受体的激动剂或拮抗剂并不影响柠檬精油的抗应激作用。丁螺环酮、DOI和米安色林在FST中阻断了柠檬精油的抗抑郁样作用，但WAY100,635没有。这些发现表明，柠檬精油的抗抑郁样作用与5-羟色胺能途径密切相关，尤其是通过5-羟色胺（1A）受体。此外，柠檬精油显著加速了海马体中多巴胺以及前额叶皮质和纹状体中5-羟色胺的代谢转换。这些结果表明，柠檬精油通过抑制与增强的5-羟色胺能神经元相关的多巴胺活性，具有抗焦虑、抗抑郁样作用。
