Liu Chuanfeng, Zhang Jiamin, Yi Fuming, Wang Junping, Wang Xiaochun, Jiang Hong, Xu Jia, Hu Yuanyang
State Key Laboratory of Virology, Laboratory of Insect Virology, College of Life Science, Wuhan University, Wuhan 430072, China.
Virus Res. 2006 Sep;120(1-2):28-35. doi: 10.1016/j.virusres.2005.09.003. Epub 2006 Jun 14.
A new insect nodavirus is isolated from Pieris rapae larvae in Wuhan city, China and tentatively designated Wuhan nodavirus (WhNV). We here report the physicochemical characterization of WhNV and determine the nucleotide sequences of its larger segment of genome, RNA1. The results show that WhNV particles are isometric, non-enveloped, with a diameter of about 29nm. The virus has a major capsid protein and a minor capsid protein with estimated molecular mass of 40 and 44kDa, respectively. WhNV RNA1 is determined to be 3149nt long, containing a 1014-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 114,608Da. The protein A shows 39 and 27% identity to its homologues in Pariacoto virus (PaV) and Striped jack necrosis nervous virus (SJNNV), respectively, but shows only 24% or less identity to its homologues in other insect Nodaviruses such as Nodamura virus (NoV), Black beetle virus (BBV), Boolarra virus (BoV) and Flock house virus (FHV). Predicted domains for six RNA-dependent RNA polymerase motifs and putative ORFs (protein B) are confirmed by sequence analysis of WhNV RNA1.
从中国武汉市的菜青虫幼虫中分离出一种新的昆虫诺达病毒，暂命名为武汉诺达病毒（WhNV）。我们在此报告WhNV的理化特性，并确定其基因组较大片段RNA1的核苷酸序列。结果表明，WhNV颗粒呈等轴对称，无包膜，直径约29nm。该病毒有一个主要衣壳蛋白和一个次要衣壳蛋白，估计分子量分别为40kDa和44kDa。确定WhNV RNA1长3149nt，含有一个1014个氨基酸的开放阅读框（ORF），编码蛋白A，计算分子量为114,608Da。蛋白A与其在帕里亚科托病毒（PaV）和条纹鲈坏死神经病毒（SJNNV）中的同源物分别有39%和27%的同一性，但与其在其他昆虫诺达病毒如野田村病毒（NoV）、黑甲虫病毒（BBV）、博拉腊病毒（BoV）和禽舍病毒（FHV）中的同源物同一性仅为24%或更低。通过对WhNV RNA1的序列分析，证实了六个依赖RNA的RNA聚合酶基序和推定的ORF（蛋白B）的预测结构域。
