印度罗氏沼虾诺达病毒（MrNV）RNA1 和 RNA2 的遗传分析。

Genetic analysis of RNA1 and RNA2 of Macrobrachium rosenbergii nodavirus (MrNV) isolated from India.

机构信息

UNESCO-MIRCEN for Marine Biotechnology, Department of Fisheries Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575002, India.

出版信息

Virus Res. 2013 May;173(2):377-85. doi: 10.1016/j.virusres.2013.01.003. Epub 2013 Jan 11.

DOI:10.1016/j.virusres.2013.01.003

PMID:23318596

Abstract

Macrobrachium rosenbergii nodavirus (MrNV) is responsible for the newly emerging catastrophic disease known as white tail disease (WTD) in M. rosenbergii. The complete sequence of RNA2 (1175 bp) and 3126 bp region of RNA1 of an Indian strain of MrNV was generated. Sequence analysis of RNA2 revealed the presence of a single ORF encoding a capsid protein of 371 amino acids with a predicted molecular mass and pI of 41.5 kDa and 8.97 respectively. RNA1 contained two ORFs, one encoding a partial RNA dependent RNA polymerase (RdRp) of length 1034 amino acids and another a B2-like protein with a length 133 amino acids. A phylogenetic analysis based on the amino acid sequence of the capsid protein, to related nodavirus sequences suggests the establishment of new genotypes within the Nodaviridae family and we suggest the name should be genus Gammanodavirus. A new reverse transcriptase-polymerase chain reaction (RT-PCR) assay has been developed and optimized for the detection of shrimp nodavirus with a sensitivity to detect up to 24 copy numbers of plasmid construct.

摘要

罗氏沼虾诺达病毒（MrNV）是引起罗氏沼虾新型暴发性疾病——白尾病（WTD）的罪魁祸首。本研究对一株来自印度的 MrNV 株系的 RNA2（1175 bp）和 RNA1 的 3126 bp 区域进行了全序列测定。RNA2 序列分析表明，其含有一个编码 371 个氨基酸的衣壳蛋白的单一开放阅读框，预测分子量和等电点分别为 41.5 kDa 和 8.97。RNA1 包含两个开放阅读框，一个编码长度为 1034 个氨基酸的部分 RNA 依赖性 RNA 聚合酶（RdRp），另一个编码长度为 133 个氨基酸的 B2 样蛋白。基于衣壳蛋白的氨基酸序列对相关诺达病毒序列进行的系统进化分析表明，在 Nodaviridae 科内建立了新的基因型，我们建议将其命名为 Gammanodavirus 属。本研究还建立并优化了一种新的逆转录-聚合酶链反应（RT-PCR）检测方法，该方法对虾诺达病毒的检测灵敏度可达 24 个质粒构建物的拷贝数。