De la Rubia J, García M, López F, Arriaga F, Marty M L, Sanz M A
Servicio de Hematología-Hemoterapia, Hospital La Fe. Valencia, España.
Sangre (Barc). 1991 Apr;36(2):89-92.
After demonstrating through animal models that progenitor cells with haemopoietic regenerative capability are present in peripheral blood, such precursor cells were also found in the mononucleated cell (MNC) fraction of human peripheral blood. It was shown that such cells increase in the recuperative period following chemotherapy. A number of studies have demonstrated that autologous infusion of such MNC achieved by cytapheresis may lead to a quick restoration of haemopoiesis. The initial results of peripheral blood MNC achievement in 18 patients with different malignancies of the blood are presented in this paper. Ninety-one cytapheresis sessions were carried out, 88 with a Fenwall CS-3000 continuous-flow machine, and the remaining three with a Haemonetics V-50 discontinuous-flow cell separator. The number of sessions per patient ranged from three to six and the harvested MNC count was 19.3 x 10(9)/L, the percentage of recovery being 56.4%. Some studies are currently in course aimed to analyse the MNC subpopulations in order to measure the progenitor-cell fraction and to establish the viability of such cells for further transplants. Since only five patients have recently been transplanted, all successfully, no definite conclusions can be drawn presently in this connection.
通过动物模型证明外周血中存在具有造血再生能力的祖细胞后，人类外周血单核细胞（MNC）组分中也发现了此类前体细胞。结果表明，此类细胞在化疗后的恢复期数量增加。多项研究表明，通过血细胞分离术进行此类MNC的自体输注可使造血功能快速恢复。本文介绍了18例不同血液恶性肿瘤患者外周血MNC采集的初步结果。共进行了91次血细胞分离术，其中88次使用Fenwall CS - 3000连续流动式血细胞分离机，其余3次使用Haemonetics V - 50间断流动式细胞分离器。每位患者的血细胞分离术次数为3至6次，采集的MNC计数为19.3×10⁹/L，回收率为56.4%。目前正在进行一些研究，旨在分析MNC亚群，以测定祖细胞比例，并确定此类细胞用于进一步移植的活力。由于最近只有5例患者成功进行了移植，目前在这方面还无法得出明确结论。
