来自昆虫地中海实蝇的细胞色素c的一级结构。

Primary structure of cytochrome c from the insect Ceratitis capitata.

作者信息

Fernández-Sousa J M, Gavilanes J G, Municio A M, Paredes J A, Pérez-Aranda A, Rodriguez R

出版信息

Biochim Biophys Acta. 1975 Jun 26;393(2):358-67. doi: 10.1016/0005-2795(75)90062-8.

Abstract

The complete amino acid sequence of cytochrome c from the Dipterous Ceratitis capitata (serie Acalypterae) has been determined by combining automatic and manual methods of sequence analysis. No overlaps between positions 79 and 80, 86 and 87, 91 and 92 as well as between 99 and 100 were obtained. The alignment of these peptides was done by homology with other sequences of cytochromes c from insects already described. Comparison with the sequences of cytochromes c of other Diptera studied so far shows three changes (positions 50, 60 and 61, according to vertebrate cytochrome c numeration) from the Acalypteran Drosophila melanogaster and five changes (positions 9, 36, 50, 60 and 61) from that of the Calypteran Haematobia irritans.

摘要

通过结合自动和手动序列分析方法，已确定了双翅目地中海实蝇（无瓣蝇类）细胞色素c的完整氨基酸序列。在第79和80位、86和87位、91和92位以及99和100位之间未发现重叠。这些肽段的比对是通过与已描述的昆虫细胞色素c的其他序列进行同源性分析完成的。与迄今研究的其他双翅目昆虫细胞色素c序列相比，与无瓣蝇类的黑腹果蝇相比有三个变化（按照脊椎动物细胞色素c的编号，位置为50、60和61），与有瓣蝇类的血蝇相比有五个变化（位置为9、36、50、60和61）。