Lyddiatt A, Boulter D
Biochem J. 1977 May 1;163(2):333-8. doi: 10.1042/bj1630333.
The amino acid sequence of locust cytochrome c was determined, although the overlap between chymotryptic and tryptic peptides at residues tyrosine-97 and leucine-98 was not observed, owing to an anomalous tryptic break duplicating the chymotryptic digestion. The molecule consists of a single polypeptide chain of 107 residues, homologous with other mitochondrial cytochromes c. In common with other known insect cytochromes c, it possesses a non-acetylated, four-residue tail at the N-terminus relative to glycine-1 of the standard alignment. A molecular phylogeny for 17 species was constructed relating the cytochrome c molecules of Schistocerca gregaria and other invertebrates with those of representative taxonomic groups. Experimental details are given in a supplementary paper deposited as Supplementary Publication SUP 50077 (24 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can obtained on the terms indicated in Biochem. J. (1977) 161, 1.
虽然由于胰蛋白酶的异常断裂重复了胰凝乳蛋白酶的消化作用，未观察到在酪氨酸-97和亮氨酸-98残基处胰凝乳蛋白酶肽段与胰蛋白酶肽段之间的重叠，但仍测定了蝗虫细胞色素c的氨基酸序列。该分子由一条含107个残基的单多肽链组成，与其他线粒体细胞色素c同源。与其他已知昆虫细胞色素c一样，相对于标准比对中甘氨酸-1而言，它在N端具有一个未乙酰化的四残基尾巴。构建了17个物种的分子系统发育树状图，将沙漠蝗和其他无脊椎动物的细胞色素c分子与代表性分类群的细胞色素c分子联系起来。实验细节载于一篇补充论文，该论文已作为补充出版物SUP 50077（共24页）存放在英国西约克郡韦瑟比市波士顿温泉村的大英图书馆出借部（邮编：LS23 7BQ），可按《生物化学杂志》（1977年）第161卷第1期所示条件从该处获取复印件。
