Lee Ju-Kyong, Park Jong-Yeol, Kim Jin-Hong, Kwon Soon-Jae, Shin Ji-Hyeon, Hong Soon-Kwan, Min Hwang-Kee, Kim Nam-Soo
Division of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Theor Appl Genet. 2006 Jun;113(1):16-22. doi: 10.1007/s00122-006-0263-9. Epub 2006 Apr 25.
We constructed a genetic linkage map with Isaac-TD, SSR, and SNAP markers in a RIL population which had been derived from a cross of waxy corn (KW7) and dent corn (Mo17). A total of 368 markers, including 241 Isaac-TD, 121 SSR, and 6 SNAP markers, were assigned to 10 linkage groups, encompassing 1687.0 cM, with an average genetic distance of 4.6 cM between markers. SSR markers were utilized as chromosome anchors, in order to assign the Isaac-TD markers to the chromosomes, and the number of markers in each of the linkage groups ranged between 22 and 49. The majority of the Isaac-TD markers were determined to have been distributed throughout the ten maize chromosomes. In linkage analysis of the Isaac-TD markers with genes of agronomic interest, six genes related with maize kernel starch biosynthesis, ae1, bt2, sh1, sh2, su1, and wx1, were analyzed and shown that they were closely linked with either the Isaac-TD or SSR markers on chromosomes of 3, 4, 5, and 9. We observed and mapped segregation-distorted markers on chromosomes 1, 5, 6, 7, 8, and 10, where these markers were clustered. The Isaac-TD or SSR markers which were closely linked with starch synthesis genes may prove useful in marker-assisted breeding programs.
我们利用Isaac-TD、SSR和SNAP标记构建了一个遗传连锁图谱，该图谱基于一个重组自交系群体，此群体源自糯玉米（KW7）和马齿玉米（Mo17）的杂交。总共368个标记，包括241个Isaac-TD、121个SSR和6个SNAP标记，被分配到10个连锁群中，覆盖1687.0厘摩，标记间平均遗传距离为4.6厘摩。利用SSR标记作为染色体锚定标记，以便将Isaac-TD标记定位到染色体上，每个连锁群中的标记数量在22到49个之间。大多数Isaac-TD标记被确定分布在十条玉米染色体上。在对Isaac-TD标记与农艺性状相关基因进行连锁分析时，分析了与玉米籽粒淀粉生物合成相关的六个基因ae1、bt2、sh1、sh2、su1和wx1，结果表明它们与3、4、5和9号染色体上的Isaac-TD或SSR标记紧密连锁。我们在1、5、6、7、8和10号染色体上观察并定位了分离扭曲标记，这些标记在这些染色体上成簇分布。与淀粉合成基因紧密连锁的Isaac-TD或SSR标记可能在标记辅助育种计划中有用。
