Castiglioni P, Ajmone-Marsan P, van Wijk R, Motto M
Istituto Sperimentale per la Cerealicoltura, Via Stezzano 24, 24100 Bergamo, Italy, IT.
Theor Appl Genet. 1999 Aug;99(3-4):425-31. doi: 10.1007/s001220051253.
We exploited the AFLP technique to saturate a RFLP linkage map derived from a maize mapping population. By using two restriction enzyme, EcoRI and PstI, differing in methylation sensitivity, both in combination with MseI, we detected 1568 bands of which 340 where polymorphic. These were added to the exitsing RFLP marker data to study the effects of incorporation of AFLPs produced by different restriction-enzyme combinations upon genetic maps. Addition of the AFLP data resulted in greater genome coverage, both through linking previously separate groups and the extension of other groups. The increase of the total map length was mainly caused by the addition of markers to telomeric regions, where RFLP markers were poorly represented. The percentage of informative loci was significantly different between the EcoRI and PstI assays. There was also evidence that PstI AFLP markers were more randomly distributed across chromosomes and chromosome regions, while EcoRI AFLP markers clustered mainly at centomeric regions. The more-random ditsribution of PstI AFLP markers on the genetic map reported here may reflect a preferential localisation of the markers in the hypomethylated telomeric regions of the chromosomes.
我们利用扩增片段长度多态性（AFLP）技术来充实一个源自玉米作图群体的限制性片段长度多态性（RFLP）连锁图谱。通过使用两种对甲基化敏感性不同的限制性内切酶EcoRI和PstI，并将它们与MseI联合使用，我们检测到1568条带，其中340条具有多态性。将这些条带添加到现有的RFLP标记数据中，以研究不同限制性内切酶组合产生的AFLP整合到遗传图谱上的效果。添加AFLP数据导致基因组覆盖范围扩大，这既通过连接先前分离的组，也通过扩展其他组来实现。总图长度的增加主要是由于在端粒区域添加了标记，而在该区域RFLP标记的数量较少。EcoRI和PstI分析中信息位点的百分比存在显著差异。也有证据表明，PstI AFLP标记在染色体和染色体区域上分布更为随机，而EcoRI AFLP标记主要聚集在着丝粒区域。本文报道的PstI AFLP标记在遗传图谱上更随机的分布可能反映了这些标记在染色体低甲基化端粒区域的优先定位。
