Hung Nguyen Ngoc, Kim Dong-Gun, Lyu Jae Il, Park Kyong-Cheul, Kim Jung Min, Kim Jin-Baek, Ha Bo-Keun, Kwon Soon-Jae
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Korea.
Department of Applied Plant Science, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Korea.
Plants (Basel). 2021 Feb 15;10(2):373. doi: 10.3390/plants10020373.
Transposable elements (TEs)-major components of eukaryotic genomes-have the ability to change location within a genome. Because of their mobility, TEs are important for genome diversification and evolution. Here, a simple rapid method, using the consensus terminal inverted repeat sequences of PONG, miniature inverted-repeat transposable element (MITE)-Tourist (M-t) and MITE-Stowaway (M-s) as target region amplification polymorphism (TE-TRAP) markers, was employed to investigate the mobility of TEs in a gamma-irradiated soybean mutant pool. Among the different TE-TRAP primer combinations, the average polymorphism level and polymorphism information content value were 57.98% and 0.14, respectively. Only the PONG sequence separated the mutant population into three major groups. The inter-mutant population variance, determined using the PONG marker (3.151 and 29%) was greater than that of the M-t (2.209 and 20%) and M-s (2.766 and 18%) markers, whereas the reverse was true for the intra-mutant population variations, with M-t and M-s values, being 15.151 (82%) and 8.895 (80%), respectively, compared with the PONG marker (7.646 and 71%). Thus, the MITE markers revealed more dynamic and active mobility levels than the PONG marker in gamma-ray irradiated soybean mutant lines. The TE-TRAP technique associated with sensitive MITEs is useful for investigating genetic diversity and TE mobilization, providing tools for mutant selection in soybean mutation breeding.
转座元件（TEs）——真核生物基因组的主要组成部分——具有在基因组内改变位置的能力。由于其移动性，TEs对基因组多样化和进化很重要。在此，采用一种简单快速的方法，以PONG、微型反向重复转座元件（MITE）-Tourist（M-t）和MITE-Stowaway（M-s）的共有末端反向重复序列作为靶区域扩增多态性（TE-TRAP）标记，来研究TEs在γ射线辐照大豆突变体库中的移动性。在不同的TE-TRAP引物组合中，平均多态性水平和多态性信息含量值分别为57.98%和0.14。只有PONG序列将突变群体分为三个主要组。使用PONG标记确定的突变群体间方差（3.151和29%）大于M-t（2.209和20%）和M-s（2.766和18%）标记，而对于突变群体内变异则相反，M-t和M-s的值分别为15.151（82%）和8.895（80%），相比之下PONG标记为7.646和71%。因此，在γ射线辐照的大豆突变系中，MITE标记显示出比PONG标记更具动态性和活跃性的移动水平。与敏感的MITEs相关的TE-TRAP技术可用于研究遗传多样性和TEs的移动性，为大豆突变育种中的突变体选择提供工具。
