Malpure Pankaj P, Shah Abhishek S, Juvekar Archana R
Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, University of Mumbai. Matunga, Mumbai 400 019, India.
Indian J Exp Biol. 2006 Jun;44(6):468-73.
Antioxidant potential of Aspergillus candidus MTCC 2202 broth filtrate extract was studied using different antioxidant models, whereas anti-inflammatory potential was studied using carrageenan-induced rat paw oedema model. The ethyl acetate extract at 1000 microg/ml showed maximum scavenging activity of the stable radical 1,1-diphenyl,2-picryl hydrazyl upto 96.65% (IC50=430.36 microg/ml) and scavenging of the radical cation, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) upto 92.25% (IC50=606.29 microg/ml) at the same concentration. The extract had good reducing power, however showed moderate inhibition for conjugated dienes and thiobarbituric acid reactive acid substances (59.56 and 51.45%). The total phenolic content of various extracts of A. candidus broth filtrate was measured and a correlation between radical scavenging activities of extracts with total phenolic content was observed. The ethyl acetate extract (125 mg/kg ip) showed significant anti-inflammatory activity in carrageenan-induced rat paw oedema model. The exhibited antioxidant activity of ethyl acetate extract of A. candidus broth filtrate was comparable with BHA and ascorbic acid, while anti-inflammatory activity was comparable with standard diclofenac sodium.
利用不同的抗氧化模型研究了白色念珠曲霉MTCC 2202肉汤滤液提取物的抗氧化潜力，而利用角叉菜胶诱导的大鼠足肿胀模型研究了其抗炎潜力。1000微克/毫升的乙酸乙酯提取物对稳定自由基1,1-二苯基-2-苦基肼的清除活性最高，可达96.65%（IC50 = 430.36微克/毫升），在相同浓度下对自由基阳离子2,2-偶氮双（3-乙基苯并噻唑啉-6-磺酸盐）的清除率可达92.25%（IC50 = 606.29微克/毫升）。该提取物具有良好的还原能力，但对共轭二烯和硫代巴比妥酸反应性酸性物质的抑制作用中等（分别为59.56%和51.45%）。测定了白色念珠曲霉肉汤滤液不同提取物的总酚含量，并观察到提取物的自由基清除活性与总酚含量之间的相关性。乙酸乙酯提取物（125毫克/千克腹腔注射）在角叉菜胶诱导的大鼠足肿胀模型中显示出显著的抗炎活性。白色念珠曲霉肉汤滤液乙酸乙酯提取物表现出的抗氧化活性与丁基羟基茴香醚和抗坏血酸相当，而抗炎活性与标准双氯芬酸钠相当。
