Vaijanathappa Jaishree, Badami Shrishailappa
Department of Pharmaceutical Chemistry, J. S. S. College of Pharmacy, Ootacamund, Tamil Nadu, India.
Planta Med. 2009 Jan;75(1):12-7. doi: 10.1055/s-0028-1088333. Epub 2008 Nov 12.
Swertiamarin, a secoiridoid isolated from the ethyl acetate extract of Enicostemma axillare, was evaluated for antiedematogenic activity using carrageenan-, formalin-, and histamine-induced paw edema methods in rats. In the carrageenan-induced method, the percentages of edema inhibition obtained after 5 h induction were 38.60, 52.50, and 45.44, respectively, for 100 and 200 mg/kg bw swertiamarin and 100 mg/kg bw of standard diclofenac sodium given orally. The activity of swertiamarin at 200 mg/kg bw was found to be superior to that of standard diclofenac sodium in all these methods. Swertiamarin was also screened for IN VITRO antioxidant activity using seven different methods: good activity was observed in ABTS and hydrogen peroxide methods, and moderate activity was observed in hydroxyl radical by deoxyribose and lipid peroxidation methods, with IC50 values of 2.83, 5.70, 52.56, and 78.33 microg/mL, respectively. The total antioxidant capacity was found to be 4.51 mM of ascorbic acid per gram of swertiamarin. Swertiamarin possesses antiedematogenic and in vitro antioxidant activities, and it may be the active constituent responsible for the anti-inflammatory activity of E. AXILLARE.
獐牙菜苦苷是从腋花獐牙菜乙酸乙酯提取物中分离得到的一种裂环烯醚萜苷，采用角叉菜胶、福尔马林和组胺诱导大鼠足肿胀法对其抗水肿活性进行了评价。在角叉菜胶诱导法中，口服100mg/kg体重和200mg/kg体重的獐牙菜苦苷以及100mg/kg体重的标准双氯芬酸钠后，诱导5小时后获得的水肿抑制百分比分别为38.60%、52.50%和45.44%。发现在所有这些方法中，200mg/kg体重的獐牙菜苦苷的活性优于标准双氯芬酸钠。还使用七种不同方法对獐牙菜苦苷的体外抗氧化活性进行了筛选：在ABTS和过氧化氢法中观察到良好活性，在脱氧核糖羟自由基法和脂质过氧化法中观察到中等活性，IC50值分别为2.83、5.70、52.56和78.33μg/mL。发现每克獐牙菜苦苷的总抗氧化能力为4.51mM抗坏血酸。獐牙菜苦苷具有抗水肿和体外抗氧化活性，它可能是腋花獐牙菜抗炎活性的活性成分。
