Mladenovska Z, Hartmann H, Kvist T, Sales-Cruz M, Gani R, Ahring B K
The Environmental Microbiology and Biotechnology Research Group, BioCentrum-DTU, Building 227, Technical University of Denmark, 2800 Lyngby, Denmark.
Water Sci Technol. 2006;53(8):59-67. doi: 10.2166/wst.2006.236.
Application of thermal treatment at 100-140 degrees C as a pretreatment method prior to anaerobic digestion of a mixture of cattle and swine manure was investigated. In a batch test, biogasification of manure with thermally pretreated solid fraction proceeded faster and resulted in the increase of methane yield. The performances of two thermophilic continuously stirred tank reactors (CSTR) treating manure with solid fraction pretreated for 40 minutes at 140 degrees C and non-treated manure were compared. The digester fed with the thermally pretreated manure had a higher methane productivity and an improved removal of the volatile solids (VS). The properties of microbial communities of both reactors were analysed. The specific methanogenic activity (SMA) test showed that both biomasses had significant activity towards hydrogen and formate, while the activity with the VFA - acetate, propionate and butyrate - was low. The kinetic parameters of the VFA conversion revealed a reduced affinity of the microbial community from the CSTR fed with thermally pre-treated manure for acetate, propionate and butyrate. The bacterial and archaeal populations identified by t-RLFP analysis of 16S rRNA genes were found to be identical in both systems. However, a change in the abundance of the species present was detected.
研究了在牛和猪粪便混合物厌氧消化之前，采用100-140摄氏度的热处理作为预处理方法。在批次试验中，经过热预处理的固体部分粪便的生物气化过程进行得更快，且甲烷产量增加。比较了两个嗜热连续搅拌釜式反应器（CSTR）的性能，一个处理在140摄氏度下预处理40分钟的固体部分粪便，另一个处理未处理的粪便。装有热预处理粪便的消化器具有更高的甲烷生产率，且挥发性固体（VS）的去除率有所提高。分析了两个反应器中微生物群落的特性。特定产甲烷活性（SMA）测试表明，两种生物质对氢气和甲酸盐都有显著活性，而对挥发性脂肪酸（VFA）——乙酸盐、丙酸盐和丁酸盐——的活性较低。VFA转化的动力学参数显示，装有热预处理粪便的CSTR中的微生物群落对乙酸盐、丙酸盐和丁酸盐的亲和力降低。通过对16S rRNA基因的t-RLFP分析确定的细菌和古菌种群在两个系统中是相同的。然而，检测到存在的物种丰度发生了变化。
