Zhang Zhenying, Liu Xiaoming, Yang Guoling, Gao Xiaorong, Jin Liji, An Lijia
Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
Mycoses. 2006 Jul;49(4):305-10. doi: 10.1111/j.1439-0507.2006.01256.x.
An attempt was made to identify the genotypes of 31 Sporothrix schenckii strains and the relationship between the genotypes, the geographic distributions and clinical manifestations using restriction fragment length polymorphic analysis. The total DNA was extracted by cetyltrimethyl ammonium bromide. The polymorphisms were detected by hybridisation of ApaI-digested Sporothrix genomic DNA with a probe amplified from the small subunit ribosomal DNA and adjacent internal transcribed spacer regions. The band patterns manifested by Southern blotting were explored to investigate the genotypes of the 31 strains of S. schenckii collected from five different areas in China. Of the data from the 31 strains of S. schenckii studied, 15 individual patterns (DNA type A-O) were recognised. Type A-C accounted for 51.61% of all the strains. The DNA typing of S. schenckii by Southern blotting was highly sensitive and highly distinguishable. We also found a significant correlation between DNA patterns and different geographic areas and clinical manifestations.
研究人员尝试运用限制性片段长度多态性分析来鉴定31株申克孢子丝菌菌株的基因型，以及这些基因型与地理分布和临床表现之间的关系。采用十六烷基三甲基溴化铵法提取总DNA。用从小亚基核糖体DNA及相邻的内部转录间隔区扩增得到的探针，与经ApaI酶切的孢子丝菌基因组DNA杂交，检测多态性。通过Southern印迹法显示的条带模式，来研究从中国五个不同地区收集的31株申克孢子丝菌的基因型。在所研究的31株申克孢子丝菌的数据中，识别出了15种个体模式（DNA型A - O）。A - C型占所有菌株的51.61%。通过Southern印迹法对申克孢子丝菌进行DNA分型具有高度敏感性和高度可区分性。我们还发现DNA模式与不同地理区域和临床表现之间存在显著相关性。
