Department of Dermatology, School of Medicine, Kanazawa Medical University, Ishikawa, Japan.
J Dermatol. 2012 Apr;39(4):362-5. doi: 10.1111/j.1346-8138.2011.01379.x. Epub 2011 Sep 28.
The complete sequences of mitochondrial DNA (mtDNA) from two strains of different genotypes, American Type Culture Collection 10268 of mtDNA type 1 and KMU2025 of mtDNA type 4, were determined. These are circular molecules, 27 125 and 26 095 bp in length, respectively. The greatest difference between the two strains was found in the region encompassed by atp9 and cox2 genes, which was amplified with polymerase chain reaction (PCR) and used for preliminary restriction fragment length polymorphism (RFLP) analysis. Eight isolates of five mtDNA types were used and RFLP patterns obtained with the restriction enzyme AseI showed that this method seems to have greater discrimination power than the other PCR-RFLP typing method using internal transcribed spacer regions of nuclear DNA.
测定了两种不同基因型菌株的线粒体 DNA（mtDNA）的完整序列，美国模式培养物集 10268 株为 mtDNA 类型 1，KMU2025 株为 mtDNA 类型 4。它们分别是 27125bp 和 26095bp 的环形分子。这两种菌株之间最大的差异存在于 atp9 和 cox2 基因所包含的区域，该区域通过聚合酶链反应（PCR）扩增，并用于初步的限制性片段长度多态性（RFLP）分析。使用了五种 mtDNA 类型的 8 个分离株，用限制性内切酶 AseI 获得的 RFLP 图谱表明，与使用核 DNA 内部转录间隔区的其他 PCR-RFLP 分型方法相比，该方法似乎具有更大的鉴别力。
