Timbuntam Walaiporn, Sriroth Klanarong, Tokiwa Yutaka
Kasetsart Agricultural and Agro-Industrial Improvement Institute, Kasetsart University, Bangkok 10900, Thailand.
Biotechnol Lett. 2006 Jun;28(11):811-4. doi: 10.1007/s10529-006-9003-0. Epub 2006 May 18.
A newly isolated sucrose-tolerant, lactic acid bacterium, Lactobacillus sp. strain FCP2, was grown on sugar-cane juice (125 g sucrose l(-1), 8 g glucose l(-1) and 6 g fructose l(-1)) for 5 days and produced 104 g lactic acid l(-1) with 90% yield. A higher yield (96%) and productivity (2.8 g l(-1 )h(-1)) were obtained when strain FCP2 was cultured on 3% w/v (25 g sucrose l(-1), 2 g glucose l(-1) and 1 g fructose l(-1)) sugar-cane juice for 10 h. Various cheap nitrogen sources such as silk worm larvae, beer yeast autolysate and shrimp wastes were also used as a substitute to yeast extract.
一种新分离出的耐蔗糖乳酸菌,乳酸杆菌属FCP2菌株,在甘蔗汁(125克蔗糖/升、8克葡萄糖/升和6克果糖/升)中培养5天,产生了104克/升乳酸,产率为90%。当FCP2菌株在3% w/v(25克蔗糖/升、2克葡萄糖/升和1克果糖/升)的甘蔗汁中培养10小时时,获得了更高的产率(96%)和生产率(2.8克/升·小时)。各种廉价的氮源,如蚕幼虫、啤酒酵母自溶物和虾废料,也被用作酵母提取物的替代品。