新型米根霉 GY18 发酵蔗糖和玉米芯水解液生产乳酸。
Efficient production of lactic acid from sucrose and corncob hydrolysate by a newly isolated Rhizopus oryzae GY18.
机构信息
Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, PO Box 294, Beijing, 100083, China.
出版信息
J Ind Microbiol Biotechnol. 2010 Nov;37(11):1137-43. doi: 10.1007/s10295-010-0761-2. Epub 2010 Jun 16.
The aim of this study is to investigate production of L-lactic acid from sucrose and corncob hydrolysate by the newly isolated R. oryzae GY18. R. oryzae GY18 was capable of utilizing sucrose as a sole source, producing 97.5 g l(-1) L-lactic acid from 120 g l(-1) sucrose. In addition, the strain was also efficiently able to utilize glucose and/or xylose to produce high yields of L-lactic acid. It was capable of producing up to 115 and 54.2 g l(-1) lactic acid with yields of up to 0.81 g g(-1) glucose and 0.90 g g(-1) xylose, respectively. Corncob hydrolysates obtained by dilute acid hydrolysis and enzymatic hydrolysis of the cellulose-enriched residue were used for lactic acid production by R. oryzae GY18. A yield of 355 g lactic acid per kg corncobs was obtained after 72 h incubation. Therefore, sucrose and corncobs could serve as potential sources of raw materials for efficient production of lactic acid by R. oryzae GY18.
本研究旨在研究新型米根霉 GY18 从蔗糖和玉米芯水解物生产 L-乳酸。米根霉 GY18 能够利用蔗糖作为唯一碳源，从 120g/L 的蔗糖中生产 97.5g/L 的 L-乳酸。此外，该菌株还能够有效地利用葡萄糖和/或木糖生产高产量的 L-乳酸。它能够分别生产高达 115 和 54.2g/L 的乳酸，葡萄糖和木糖的产率分别高达 0.81gg-1 和 0.90gg-1。通过稀酸水解和纤维素富集残渣的酶解获得的玉米芯水解物用于米根霉 GY18 生产乳酸。经过 72 小时培养，每公斤玉米芯可获得 355g 乳酸。因此，蔗糖和玉米芯可以作为米根霉 GY18 高效生产乳酸的潜在原料。
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