Holtmann Dirk, Schrader Jens, Sell Dieter
Karl-Winnacker-Institut der DECHEMA eV, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany.
Biotechnol Lett. 2006 Jun;28(12):889-96. doi: 10.1007/s10529-006-9021-y. Epub 2006 May 31.
The microbial activity of different microorganisms was determined by means of an electrochemical bioactivity sensor (BAS). The BAS is based on a biofuel cell and was used for analytical purposes. Online determination of microbial activity using the BAS demonstrated that when different microorganisms with different metabolic pathways were cultivated, a distinct activity signal was detectable with all organisms applied. Furthermore, the results permitted a quantitative comparison of the BAS signals. Among other findings it was shown that the quotient of the BAS signal and the utilized glucose varied from 0.16-29.08 mV g(-1), the quotient of the maximum BAS signal and the released energy of the reaction exhibited a lower variation of 0.07-0.19 mV kJ(-1). Furthermore it was demonstrated that the highest BAS signals could be measured during anaerobic E. coli fermentations, the reason being the formation of electroactive fermentation products, such as formic acid and H(2).
采用电化学生物活性传感器（BAS）测定不同微生物的微生物活性。该BAS基于生物燃料电池，用于分析目的。使用BAS在线测定微生物活性表明，当培养具有不同代谢途径的不同微生物时，在所应用的所有生物体中均可检测到明显的活性信号。此外，结果允许对BAS信号进行定量比较。除其他发现外，还表明BAS信号与所利用葡萄糖的商在0.16 - 29.08 mV g(-1)之间变化，最大BAS信号与反应释放能量的商变化较小，为0.07 - 0.19 mV kJ(-1)。此外，还证明在厌氧大肠杆菌发酵过程中可测量到最高的BAS信号，原因是形成了电活性发酵产物，如甲酸和H(2)。
